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AB251093

Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal JNK1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Dog, Cow, Xenopus tropicalis, Recombinant fragment, Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

6 Images
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • WB

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 30s

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • IP

Supplier Data

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : K562 whole cell extract 10 μg (Input).

Lane 2 : ab191603 IP in K562 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>)

Observed band size: 46 kDa,54 kDa

false

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • WB

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>) at 1/2000 dilution

Lane 1:

Full length human JNK1 recombinant protein at 0.01 µg

Lane 2:

Full length human JNK2 recombinant protein at 0.01 µg

Lane 3:

Full length human JNK3 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa,71 kDa

false

Exposure time: 3min

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • WB

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 1min

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • WB

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>) at 1/2000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 3min

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)
  • WB

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (AB251093)

This data was developed using ab191603, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (<a href='/en-us/products/primary-antibodies/jnk1-jnk3-antibody-epr16797-194-ab191603'>ab191603</a>) at 1/2000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cells) whole cell lysate at 10 µg

Lane 2:

Xenopus (X. tropicalis) muscle lysates at 10 µg

Lane 3:

MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Lane 4:

MDBK (Bovine kidney cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16797-194

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Cow, Dog, Human, Xenopus tropicalis, Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Xenopus tropicalis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251093 is the carrier-free version of ab191603.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Jun N-terminal kinases 1 and 3 (JNK1 and JNK3) are members of the mitogen-activated protein kinase (MAPK) family also known as Stress-Activated Protein Kinases (SAPKs). JNK1 has a molecular mass of approximately 46-54 kDa while JNK3 has a mass of about 54 kDa. JNK1 is expressed in most tissues with high levels in heart brain and leukocytes whereas JNK3 is mainly expressed in the brain but also found in the testes and heart. These kinases play a role in signaling cascades that respond to stress stimuli such as cytokines ultraviolet radiation and reactive oxygen species.
Biological function summary

JNK1 and JNK3 regulate transcription factors and influence gene expression to modulate cellular processes. They are part of a complex network that includes other proteins like MAP kinase kinases (MKKs) which activate JNKs through dual phosphorylation. JNK1 and JNK3 control processes such as cell proliferation apoptosis and differentiation. Their activation can trigger the phosphorylation of c-Jun a component of the Activator Protein 1 (AP-1) transcription factor complex influencing cell fate decisions.

Pathways

JNK1 and JNK3 are involved in important signaling pathways such as the MAPK signaling pathway and the apoptosis signaling pathway. These pathways interact with other proteins like MKK4 and MKK7 which act upstream to activate JNKs. The JNK pathway helps the cell respond to stress and regulate apoptosis interacting with proteins such as Bcl-2 which is involved in many cellular responses including cell survival and death.

JNK1 and JNK3 have been associated with conditions such as neurodegenerative diseases and cancer. Abnormal activation of these kinases is linked to Alzheimer’s disease which involves protein misfolding and synaptic dysfunction and to various cancers due to their role in controlling cell death and proliferation. In these contexts they interact with disease-related proteins like tau in Alzheimer's disease and p53 in cancer affecting disease progression and therapeutic responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
See full target information MAPK8

Additional targets

MAPK10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu
View all publications
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