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AB215208

Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763]

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(16 Publications)

Rabbit Recombinant Monoclonal JNK1 phospho Y185 + T183 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 16 publications.

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

4 Images
Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)
  • IP

Supplier Data

Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)

JNK1 (phospho T183 + Y185) was immunoprecipitated from 0.35mg of HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate with ab215208 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215208 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate 10 μg (Input).

Lane 2 : ab215208 IP in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215208 in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

Predicted band size: 48 kDa

true

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)
  • WB

Supplier Data

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)

Blocking/Dilution : 5% NFDM/TBST.

Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID : 15386344).

All lanes:

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

Lane 1:

Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate at 10 µg

Lane 2:

HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

true

Exposure time: 1min

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)
  • WB

Supplier Data

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)

Blocking/Dilution : 5% NFDM/TBST.

Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID : 15386344).

All lanes:

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

Lane 1:

Untreated U-2 OS (human bone osteosarcoma epithelial cell line) cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate at 10 µg

Lane 2:

U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

true

Dot Blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)
  • Dot

Supplier Data

Dot Blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (AB215208)

Dot blot analysis of JNK1 (phospho T183+ Y185) labeled with ab215208 at 1/1000 dilution.

Lane 1 : JNK1 (phospho T183/Y185) peptide.
Lane 2 : JNK1 (phospho T183) peptide.
Lane 3 : JNK1 (phospho Y185) peptide.
Lane 4 : JNK1 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Based on sequence homology this antibody also reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20763

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on sequence homology this antibody cross reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.
Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
See full target information MAPK8 phospho Y185 + T183
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1565392 PubMed41035878

2025

Gastric mucosal proteomic analysis reveals the role of the TGF- signaling pathway in gastritis associated with infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjie Zhu,Lijuan Xiong,Yun He,Chaoqin Sun,Fei Mo

Clinical and translational medicine 15:e70273 PubMed40070022

2025

Integrated spatial multi-omics profiling of Fusobacterium nucleatum in breast cancer unveils its role in tumour microenvironment modulation and cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhao,Rui An,Yilei Ma,Shaobo Yu,Yuzhen Gao,Yanzhong Wang,Haitao Yu,Xinyou Xie,Jun Zhang

Frontiers in oncology 14:1433256 PubMed39717750

2024

Neuroblastoma response to RAS-MAPK inhibitors and APR-246 (eprenetapopt) co-treatment is dependent on SLC7A11.

Applications

Unspecified application

Species

Unspecified reactive species

Vid Mlakar,Ina Oehme,Laurence Lesne,Sara Najafi,Marc Ansari,Fabienne Gumy-Pause

Journal of biochemical and molecular toxicology 38:e23682 PubMed38462752

2024

CLDN6 inhibited cellular biological function of nonsmall cell lung cancer cells through suppressing aerobic glycolysis via the RIP1/ASK1/JNK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Guo,Jianying Li,Yu Dong,Humei Gao,Peng Wang

Allergologia et immunopathologia 52:60-67 PubMed38459892

2024

YBX-1 alleviates sepsis-stimulated lung epithelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Lu,Shouqian Dai,Pengfei Li,Yuqian Zhou,Feng Xu

Discover oncology 15:45 PubMed38383815

2024

TRAIL predisposes non-small cell lung cancer to ferroptosis by regulating ASK-1/JNK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Liu,Huiqian Deng,Mi Huang,Wei Zhou,Yilin Yang

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

Frontiers in pharmacology 14:1138858 PubMed37388447

2023

Network pharmacology-based approach to explore the underlying mechanism of sinomenine on sepsis-induced myocardial injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Linggang Sun,Zhiyun Chen,Yunjie Ni,Zhengfei He

Molecular medicine reports 24: PubMed34713293

2021

Biological evaluation of linalool on the function of blood vessels.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyu Liang,Yan Zhong,Xinmei Li,Yingying Xiao,Yu Wu,Pingchang Xie

Experimental and therapeutic medicine 22:1401 PubMed34650647

2021

DUSP4 alleviates LPS-induced chondrocyte injury in knee osteoarthritis via the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengnan Li,Bojie Chen
View all publications
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