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Rabbit Recombinant Monoclonal JNK1 phospho Y185 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 71 publications.


Images

Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572), expandable thumbnail
  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572), expandable thumbnail
  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572), expandable thumbnail
  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572), expandable thumbnail
  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/70
Notes

For unpurified use at 1/20.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

For unpurified use at 1/5000 - 1/20000.

Species
Rat
Dilution info
1/5000
Notes

For unpurified use at 1/5000 - 1/20000.

Species
Human
Dilution info
1/5000
Notes

For unpurified use at 1/5000 - 1/20000.

Associated Products

Select an associated product type

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Target data

Function

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed:28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed:21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed:21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed:21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed:21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed:18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed:16581800, PubMed:17296730, PubMed:20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed:22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed:27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed:28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed:34048572). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Additional Targets

MAPK10 phospho Y223, MAPK10 phospho Y223, MAPK9 phospho Y185

Alternative names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

Recommended products

Rabbit Recombinant Monoclonal JNK1 phospho Y185 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 71 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1597Y
Purification technique
Affinity purification Protein A
Specificity

Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The JNK protein family also known as c-Jun N-terminal kinases consists of JNK1 JNK2 and JNK3. These proteins are mitogen-activated protein kinases (MAPKs) that play a role in transmitting extracellular signals to the cell nucleus. JNK1 has a mass of approximately 54 kDa and is ubiquitously expressed in various tissues. JNK2 is similar in mass and expression while JNK3 is primarily found in the brain heart and testes. These proteins can phosphorylate transcription factors such as c-Jun impacting gene expression processes.

Biological function summary

The JNK proteins are involved in controlling cellular events like proliferation differentiation and apoptosis. They act as part of the MAPK signaling complex and involve scaffolding proteins that help organize these signaling pathways. In the cellular context JNK proteins are activated in response to stress signals like UV irradiation inflammatory cytokines and growth factors. This activation leads to diverse cellular outcomes depending on the specific JNK isoform and cell type.

Pathways

The JNK proteins are important in the MAPK signaling pathways and play a role in the AP-1 transcription factor regulation. They interact with proteins such as MEK4 and MEK7 which are upstream kinases that activate JNK through phosphorylation. The JNK signaling arm is distinct from the ERK and p38 MAPK pathways each having specific roles in cellular response modulation.

Associated diseases and disorders

The JNK proteins are implicated in conditions like neurodegenerative diseases and cancer. In Alzheimer's disease for example JNK3 contributes to neuronal cell death while JNK inhibitors like SP600125 have been explored for therapeutic potential. In cancer JNK proteins can influence tumor progression through the regulation of apoptosis and their activity may intersect with other kinases like p53 further complicating disease pathways. JNK's role in diseases makes it a critical target for developing inhibitors reflected in studies around compounds like JNK inhibitor 8 and related molecules.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572), expandable thumbnail

    Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10μg)
    Lane 2 (+): ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 46, 54 kDa

    All lanes: Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572), expandable thumbnail

    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Blocking/Diluting buffer: 5% NFDM/TBST

    Lanes 1 - 6: Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/5000 dilution

    Lane 7: Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 20 µg

    Lane 5: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Lane 6: C6 (Rat glial tumor glial cell) treated with 40J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 46 kDa, 54 kDa

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572), expandable thumbnail

    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) Western blot staining using rabbit Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody

    All lanes: Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/2000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour, whole cell lysates. Then the membrane was incubated with alkaline phosphatase at 15 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with lambda phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 46 kDa, 54 kDa

    Exposure time: 30s

  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572), expandable thumbnail

    Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Lane 1: Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
    Lane 2: Human JNK1+JNK2+JNK3 non-phospho pepetide

    Stained with unpurified ab76572 at a 1:1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at a 1:100,000 dilution.

    Exposure: 3 minutes

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) western blot using anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] ab76572. Publication image and figure legend from Zhao, T. M., Wang, Y., et al., 2020, Front Pharmacol, PubMed 32362825.


    ab76572 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76572 please see the product overview.

    Effect of bicyclol on hepatic AMPK, mTOR, and MAPK protein expressions at 24 and 48 h after CCl4 exposure. Mice were intraperitoneally injected a mixture of CCl4 (50%) and oil (50%) at a dose of 2 ml/kg body weight. Mice were given bicyclol 200 mg/kg by gavage for three times in 1 day 1 h prior to CCl4 challenge. Results are presented as mean ± SD for five mice per group. Significantly different ($$p < 0.01, $$$p < 0.001) from the CCl4 24 h group. Significantly different (&p < 0.05, &&p < 0.01) from the CCl4 48 h group.

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