AB76572

Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]

Name: Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) | Abcam
Brand: Abcam Limited
SKU: AB76572
Price: 240 USD
Availability: InStock
Rating: 4 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(91 Publications)

Rabbit Recombinant Monoclonal JNK1 phospho Y185 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 91 publications.

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c, JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a, JNK3, JNK3A, PRKM10, SAPK1B, MAPK10, Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b

5 Images

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Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10μg)
Lane 2 (+) : ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 46, 54 kDa

All lanes:

Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

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Unknown

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Blocking/Diluting buffer : 5% NFDM/TBST

Lanes 1 - 6:

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/5000 dilution

Lane 7:

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) treated with 40J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 46 kDa,54 kDa

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Lab

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour, whole cell lysates. Then the membrane was incubated with alkaline phosphatase at 15 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with lambda phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 46 kDa,54 kDa

false

Exposure time: 30s

Unknown

Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Lane 1 : Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
Lane 2 : Human JNK1+JNK2+JNK3 non-phospho pepetide

Stained with unpurified ab76572 at a 1 : 1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1 : 100,000 dilution.

Exposure : 3 minutes

CiteAb

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) western blot using anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] ab76572. Publication image and figure legend from Zhao, T. M., Wang, Y., et al., 2020, Front Pharmacol, PubMed 32362825.

ab76572 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76572 please see the product overview.

Effect of bicyclol on hepatic AMPK, mTOR, and MAPK protein expressions at 24 and 48 h after CCl4 exposure. Mice were intraperitoneally injected a mixture of CCl4 (50%) and oil (50%) at a dose of 2 ml/kg body weight. Mice were given bicyclol 200 mg/kg by gavage for three times in 1 day 1 h prior to CCl4 challenge. Results are presented as mean ± SD for five mice per group. Significantly different ($$p < 0.01, $$$p < 0.001) from the CCl4 24 h group. Significantly different (&p < 0.05, &&p < 0.01) from the CCl4 48 h group.

false

Carrier free

Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1597Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "For unpurified use at 1/20.", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/5000 - 1/20000." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/5000 - 1/20000." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/5000 - 1/20000." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JNK protein family also known as c-Jun N-terminal kinases consists of JNK1 JNK2 and JNK3. These proteins are mitogen-activated protein kinases (MAPKs) that play a role in transmitting extracellular signals to the cell nucleus. JNK1 has a mass of approximately 54 kDa and is ubiquitously expressed in various tissues. JNK2 is similar in mass and expression while JNK3 is primarily found in the brain heart and testes. These proteins can phosphorylate transcription factors such as c-Jun impacting gene expression processes.

Biological function summary

The JNK proteins are involved in controlling cellular events like proliferation differentiation and apoptosis. They act as part of the MAPK signaling complex and involve scaffolding proteins that help organize these signaling pathways. In the cellular context JNK proteins are activated in response to stress signals like UV irradiation inflammatory cytokines and growth factors. This activation leads to diverse cellular outcomes depending on the specific JNK isoform and cell type.

Pathways

The JNK proteins are important in the MAPK signaling pathways and play a role in the AP-1 transcription factor regulation. They interact with proteins such as MEK4 and MEK7 which are upstream kinases that activate JNK through phosphorylation. The JNK signaling arm is distinct from the ERK and p38 MAPK pathways each having specific roles in cellular response modulation.

The JNK proteins are implicated in conditions like neurodegenerative diseases and cancer. In Alzheimer's disease for example JNK3 contributes to neuronal cell death while JNK inhibitors like SP600125 have been explored for therapeutic potential. In cancer JNK proteins can influence tumor progression through the regulation of apoptosis and their activity may intersect with other kinases like p53 further complicating disease pathways. JNK's role in diseases makes it a critical target for developing inhibitors reflected in studies around compounds like JNK inhibitor 8 and related molecules.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

See full target information MAPK8 pY185

Additional targets

MAPK10 phospho Y223,

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Publications (91)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565375

2025

Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Ding,Yuan Sun,Wulipan Tuohudaali,Chenyang Li,Yuhan Yao,Jun Zhao

Journal of biochemical and molecular toxicology 39:e70268 PubMed40269590

2025

N-Acety-L-Cysteine Alleviates Isoflurane-Triggered Neuronal Cell Parthanatos by Suppressing Reactive Oxygen Species Accumulation Through the Induction of c-Jun N-Terminal Kinase Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liu,Ya Liu,Xuedong Wang,Ming Liu,Yingying Wang,Chunsheng Feng,Meihua Piao

British journal of pharmacology 182:2897-2913 PubMed40097259

2025

TLR2 activates AP-1 to facilitate CTGF transcription and stimulate doxorubicin-induced myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lang Hong,Xinyong Cai,Yuliang Zhan,Songtao Liu,Pengtao Zou,Yanmei Chen,Liang Shao

Veterinary research 55:93 PubMed39075605

2024

HbpA from Glaesserella parasuis induces an inflammatory response in 3D4/21 cells by activating the MAPK and NF-κB signalling pathways and protects mice against G. parasuis when used as an immunogen.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yang,Yiwen Zhang,Qin Zhao,Senyan Du,Xiaobo Huang,Rui Wu,Qigui Yan,Xinfeng Han,Yiping Wen,San-Jie Cao

Immunity, inflammation and disease 12:e1077 PubMed38722267

2024

Tectorigenin inhibits inflammatory responses in murine inflammatory bowel disease and LPS-stimulated macrophages via inactivating MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Huang,Sanhui Tang,Yanghong Zhou,Yi Cai

Cell communication and signaling : CCS 22:160 PubMed38439009

2024

Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaole Peng,Tianhao Wang,Qing Wang,Yuhu Zhao,Hao Xu,Huilin Yang,Ye Gu,Yunxia Tao,Bangsheng Yan,Yaozeng Xu,Dechun Geng

Frontiers in pharmacology 15:1286546 PubMed38389927

2024

Acacetin inhibits inflammation by blocking MAPK/NF-κB pathways and NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Bu,Yeledan Mahan,Shengnan Zhang,Xuanxia Wu,Xiaoling Zhang,Ling Zhou,Yanmin Zhang

Journal of neuropathology and experimental neurology 83:194-204 PubMed38230623

2024

YTHDF2-regulated matrilin-3 mitigates post-reperfusion hemorrhagic transformation in ischemic stroke via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hanze Chen,Siping Guo,Runnan Li,Lihui Yang,Rui Wang,Yasi Jiang,Yonggang Hao

Experimental and therapeutic medicine 27:8 PubMed38223326

2024

Butorphanol inhibits ferroptosis to attenuate PC12 cell injury by blocking JNK/p38 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Ji,Qing She,Ping Zhou,Yibin Qin

Nutrients 16: PubMed38201995

2024

Protective Effect of Lam. (Jackfruit) Polysaccharides on Liver Injury Induced by Cyclophosphamide in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Cheng,Yifan Zheng,Gang Wu,Lehe Tan,Fei Xu,Yanjun Zhang,Xiaoai Chen,Kexue Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]

Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (AB76572)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (91)

Product promise