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AB239886

Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az

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(2 Publications)

Rabbit Recombinant Monoclonal JNK1 phospho Y185 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

3 Images
Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)
  • IP

Unknown

Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)

Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10μg)
Lane 2 (+) : ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 46, 54 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572).

All lanes:

Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (<a href='/en-us/products/primary-antibodies/jnk1-phospho-y185-jnk2-phospho-y185-jnk3-phospho-y223-antibody-ep1597y-ab76572'>ab76572</a>)

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Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)
  • WB

Unknown

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572).

Lanes 1 - 6:

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (<a href='/en-us/products/primary-antibodies/jnk1-phospho-y185-jnk2-phospho-y185-jnk3-phospho-y223-antibody-ep1597y-ab76572'>ab76572</a>) at 1/5000 dilution

Lane 7:

Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (<a href='/en-us/products/primary-antibodies/jnk1-phospho-y185-jnk2-phospho-y185-jnk3-phospho-y223-antibody-ep1597y-ab76572'>ab76572</a>)

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) treated with 40J/m2 UV-C then recovery for 30 minutes whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 46 kDa,54 kDa

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Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)
  • Dot

Unknown

Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] - BSA and Az (AB239886)

Lane 1 : Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
Lane 2 : Human JNK1+JNK2+JNK3 non-phospho pepetide

Stained with ab76572 at a 1 : 1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1 : 100,000 dilution.

Exposure : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76572).

  • Unconjugated

    Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1597Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

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Reactivity data

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Product details

ab239886 is the carrier-free version of ab76572.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JNK protein family also known as c-Jun N-terminal kinases consists of JNK1 JNK2 and JNK3. These proteins are mitogen-activated protein kinases (MAPKs) that play a role in transmitting extracellular signals to the cell nucleus. JNK1 has a mass of approximately 54 kDa and is ubiquitously expressed in various tissues. JNK2 is similar in mass and expression while JNK3 is primarily found in the brain heart and testes. These proteins can phosphorylate transcription factors such as c-Jun impacting gene expression processes.
Biological function summary

The JNK proteins are involved in controlling cellular events like proliferation differentiation and apoptosis. They act as part of the MAPK signaling complex and involve scaffolding proteins that help organize these signaling pathways. In the cellular context JNK proteins are activated in response to stress signals like UV irradiation inflammatory cytokines and growth factors. This activation leads to diverse cellular outcomes depending on the specific JNK isoform and cell type.

Pathways

The JNK proteins are important in the MAPK signaling pathways and play a role in the AP-1 transcription factor regulation. They interact with proteins such as MEK4 and MEK7 which are upstream kinases that activate JNK through phosphorylation. The JNK signaling arm is distinct from the ERK and p38 MAPK pathways each having specific roles in cellular response modulation.

The JNK proteins are implicated in conditions like neurodegenerative diseases and cancer. In Alzheimer's disease for example JNK3 contributes to neuronal cell death while JNK inhibitors like SP600125 have been explored for therapeutic potential. In cancer JNK proteins can influence tumor progression through the regulation of apoptosis and their activity may intersect with other kinases like p53 further complicating disease pathways. JNK's role in diseases makes it a critical target for developing inhibitors reflected in studies around compounds like JNK inhibitor 8 and related molecules.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
See full target information MAPK8 phospho Y185

Additional targets

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:869365 PubMed35967407

2022

ILC2s expanded by exogenous IL-33 regulate CD45+CD11b+F4/80high macrophage polarization to alleviate hepatic ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Ming Zhang,Xiao-Jie Chen,Shi-Peng Li,Jin-Ming Zhang,Jie Sun,Liu-Xin Zhou,Guang-Peng Zhou,Bin Cui,Li-Ying Sun,Zhi-Jun Zhu

Folia neuropathologica 59:50-66 PubMed33969677

2021

Inhibition of ASC enhances the protective role of salvianolic acid A in traumatic brain injury via inhibition of inflammation and recovery of mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Chao Xu,Kunhu Zhang,Jianjun Shi,Zhenyang Liu,Xinxing Wang,Min Guo,Jiannan Lv,Xinmin Ding
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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