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AB76125

Anti-JNK2 antibody [EP1595Y]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

5

(1 Review)

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(57 Publications)

Anti-JNK2 antibody [EP1595Y] (ab76125) is a rabbit monoclonal antibody detecting JNK2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ELISA. Suitable for Human,.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2009

View Alternative Names

JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK2 antibody [EP1595Y] (AB76125)

ab76125 at 1/100 dilution staining JNK2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-JNK2 antibody [EP1595Y] (AB76125)

Overlay histogram showing HeLa cells stained with ab76125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76125, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunoprecipitation - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • IP

Unknown

Immunoprecipitation - Anti-JNK2 antibody [EP1595Y] (AB76125)

JNK2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit monoclonal to JNK2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76125.

Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

Band : 48kDa; JNK2

All lanes:

Immunoprecipitation - Anti-JNK2 antibody [EP1595Y] (ab76125)

Predicted band size: 48 kDa

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Exposure time: 20min

ELISA - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • ELISA

Unknown

ELISA - Anti-JNK2 antibody [EP1595Y] (AB76125)

ELISA analysis of Human JNK2 recombinant protein at 250 ng/mL with ab76125. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • WB

Lab

Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)

Lanes 1-3 : Merged signal (red and green). Green - ab76125 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

ab76125 Anti-JNK2 antibody [EP1595Y] was shown to specifically react with JNK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266355 (knockout cell lysate ab257527) was used. Wild-type and JNK2 knockout samples were subjected to SDS-PAGE. ab76125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK9 (JNK2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mapk9-jnk2-knockout-hek-293t-cell-line-ab266355'>ab266355</a>)

Lane 2:

MAPK9 knockout HEK293T cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • WB

Unknown

Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : JNK2 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab76125 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab76125 was shown to specifically react with JNK2 when JNK2 knockout samples were used. Wild-type and JNK2 knockout samples were subjected to SDS-PAGE. ab76125 and ab8245 (loading control to GAPDH) were diluted 1/2500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125)

Predicted band size: 48 kDa

false

Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)
  • WB

Unknown

Western blot - Anti-JNK2 antibody [EP1595Y] (AB76125)

All lanes:

Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125) at 1/50000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit-HRP at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1595Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), IP, ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-JNK2 antibody [EP1595Y] (ab76125) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA in Human, samples.

What is the molecular weight of JNK2?
Anti-JNK2 [EP1595Y] (ab76125) specifically detects a band for JNK2 (UniProt: P45984) at a molecular weight of 48kDa.

Trusted by the scientific community
Anti-JNK2 [EP1595Y] (ab76125) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-JNK2 antibody [EP1595Y] (ab76125) has been confirmed by Western blot testing in MAPK9 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EP1595Y] also available for your convenience: ab76125, Carrier free - ab227986, PE - ab306259, APC - ab306260, HRP - ab306261, Alkaline Phosphatase - ab308659, Alexa Fluor® 488 - ab309634, Alexa Fluor® 647 - ab310000, Alexa Fluor® 594 - ab310363, Alexa Fluor® 555 - ab311886, Alexa Fluor® 568 - ab312355, Alexa Fluor® 750 - ab321718

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Jun N-terminal kinase 2 also known as JNK2 is a member of the MAPK (mitogen-activated protein kinase) family. JNK2 plays a significant role in transmitting signals within cells. It is a protein with a mass of approximately 48 kDa and exists in various tissues including the brain heart and liver. JNK2 is ubiquitously expressed and has two main isoforms produced by alternative splicing. These isoforms are involved in different biological functions emphasizing the protein's versatility.
Biological function summary

C-Jun N-terminal kinase 2 is key in regulating processes such as cell growth apoptosis and differentiation. JNK2 is part of the MAP kinase signal transduction pathways and forms interactions with several proteins including the scaffolding proteins known as JNK-interacting proteins (JIPs). These complexes help coordinate the response of JNK2 in cellular stress and inflammatory responses. JNK2 is also critical in modulating the expression of genes by activating transcription factors such as c-Jun and ATF2.

Pathways

JNK2 operates within the MAPK signaling pathway by integrating various upstream signals to exert effects on gene expression. JNK2 phosphorylates and activates transcription factors playing an important role in cellular responses to stress. It is closely connected to other proteins within the pathway such as JNK1 and JNK3 together contributing to the complex regulation of stress-induced apoptosis and pro-inflammatory responses. These interactions highlight JNK2's essential function across multiple signaling networks.

Research connects c-Jun N-terminal kinase 2 to both cancer and neurodegenerative diseases. Its role in controlling apoptosis and cell proliferation links JNK2 to tumor progression where abnormal JNK2 activity can lead to oncogenesis. Additionally in neurodegenerative diseases like Alzheimer's dysregulated JNK2 signaling may accelerate neuronal death. JNK2's association with other proteins involved in these disorders such as amyloid precursor protein in Alzheimer's disease highlights its influence in pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 10376527). In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3 (PubMed : 15805466). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1 (PubMed : 17525747, PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells (PubMed : 19290929). Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels (PubMed : 19290929). Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption (PubMed : 20595622). When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway (PubMed : 19675674). Participates also in neurite growth in spiral ganglion neurons (By similarity). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. MAPK9 isoforms display different binding patterns : alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
See full target information MAPK9
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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:95092 PubMed40093279

2025

O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: and study.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Ke Wu,Min Liu,Hong-Ling Zhou,Xiang He,Jing Wei,Wei-Han Hua,Hui-Jing Li,Qiang-Hua Yuan,Yun-Fei Xie

Nature communications 16:1240 PubMed39890781

2025

Proteomic profiling identifies muscle-invasive bladder cancers with distinct biology and responses to platinum-based chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

A Contreras-Sanz,G L Negri,M J Reike,H Z Oo,J M Scurll,S E Spencer,K Nielsen,K Ikeda,G Wang,C L Jackson,S Gupta,M E Roberts,D M Berman,R Seiler,G B Morin,P C Black

OncoTargets and therapy 17:471-487 PubMed38895133

2024

Unraveling the Mechanism of Curculiginis Rhizoma in Suppressing Cisplatin Resistance in Non-Small Cell Lung Cancer: An Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Huang,Meng Wang,Baochen Zhu,Yu Hao,Ruoyu Gao,Wenhui Liu,Jiaojiao Cheng,Guodong Hua,Chunmiao Xue

Biomolecules & biomedicine 24:323-336 PubMed37540586

2024

CCNI2 promotes pancreatic cancer through PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyang Hu,Wenzhi Zhang,Changsheng Zhang,Chonghui Li,Ning Zhang,Ke Pan,Xinlan Ge,Tao Wan

Aging and disease 15:369-389 PubMed37307823

2024

Cancer-Associated Fibroblasts Promote Lymphatic Metastasis in Cholangiocarcinoma via the PDGF-BB/PDGFR-β Mediated Paracrine Signaling Network.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yan,Gang Xiao,Caini Yang,Qinqin Liu,Cui Lv,Xianhuan Yu,Ziyu Zhou,Shusheng Lin,Zhenhua Bai,Haoming Lin,Rui Zhang,Chao Liu

Iranian journal of public health 52:2412-2416 PubMed38106830

2023

Lncrna FGFR3-AS1 Is a Prognostic Indicator for Ovarian Cancer and Induces Cell Proliferation and Hinders Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyang Zhang,Lili Sun,Tieshuang Chen,Yuanyuan Yue,Lin Zhao,Dan Zhang

Cell cycle (Georgetown, Tex.) 22:2097-2112 PubMed37974357

2023

Depleting ANTXR1 suppresses glioma growth via deactivating PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyang Zhou,Aijun Liang,Jianzhong Zhang,Jingxing Leng,Bin Xi,Bin Zhou,Yu Yang,Ronglan Zhu,Liangchen Zhong,Xingxing Jiang,Dengfeng Wan

Cells 12: PubMed37759456

2023

Long-Term Alcohol-Activated c-Jun N-terminal Kinase Isoform 2 Preserves Cardiac Function but Drives Ca-Triggered Arrhythmias.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Ricchiuti,Kurtis Chenoweth,Xianlong Gao,Dan J Bare,Jiajie Yan,Xun Ai

Journal of gastrointestinal oncology 13:2447-2457 PubMed36388700

2022

TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yu,Jieping Huang,Hui Yu,Jingping Lin,Fang Fan,Ruixiang Xie,Yongshi Shen,Kangni Lin,Yong Ye,Jinsen Weng

Allergologia et immunopathologia 50:1-9 PubMed36335439

2022

PHLDA1 knockdown inhibits inflammation and oxidative stress by regulating JNK/ERK pathway, and plays a protective role in sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Gong,Wenmei Liang,Qinju Lu,Jing Zhang
View all publications
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Product promise

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