AB118221

Anti-JOSD1 antibody

Name: Anti-JOSD1 antibody (ab118221) | Abcam
Brand: Abcam Limited
SKU: AB118221

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(4 Publications)

Rabbit Polyclonal JOSD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human JOSD1 aa 1-200.

View Alternative Names

JSPH1, KIAA0063, JOSD1, Josephin-1, Josephin domain-containing protein 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JOSD1 antibody (AB118221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JOSD1 antibody (AB118221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labeling JOSD1 with ab118221 at a dilution of 1/100.

Unknown

Western blot - Anti-JOSD1 antibody (AB118221)

All lanes:

Western blot - Anti-JOSD1 antibody (ab118221) at 1/500 dilution

Lane 1:

HepG2 cell lysate

Lane 2:

A549 cell lysate

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human JOSD1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q15040

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab118221 is purified by a protein affinity column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JOSD1 also known as Josephin domain-containing protein 1 functions as a deubiquitinating enzyme. It specifically removes ubiquitin from protein substrates which regulates protein degradation localization and activity in cells. The protein mass is approximately 45 kDa. JOSD1 is expressed in various tissues with significant levels seen in the brain liver and kidney. By modulating the ubiquitination status of substrates JOSD1 influences cellular processes like signal transduction and response to stress.

Biological function summary

This protein modifies the stability and activity of other proteins by removing ubiquitin molecules. It acts as part of a larger deubiquitinase complex which interacts with protein networks to maintain cellular homeostasis. Through its enzyme activity JOSD1 contributes to the regulation of processes such as protein quality control and cell cycle progression.

Pathways

Scientists have placed JOSD1 in the ubiquitin-proteasome system an important pathway responsible for protein turnover in cells. It has connections with other proteins like USP7 and USP9X in this pathway reflecting its coordination role in protein deubiquitination. JOSD1 also participates in the endoplasmic reticulum-associated degradation (ERAD) pathway helping to eliminate misfolded proteins important for maintaining protein folding homeostasis within the cell.

Abnormal activity of JOSD1 can connect to neurodegenerative disorders such as Alzheimer's disease where protein misfolding and aggregation play significant roles. JOSD1's interaction with proteins like tau and amyloid precursor protein suggests its potential to influence disease progression. Additionally research associates JOSD1 with liver cancer where dysregulated ubiquitin signaling may contribute to tumor development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.

See full target information JOSD1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:405 PubMed39284830

2024

Deubiquitinase JOSD1 tempers hepatic proteotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Saheli Chowdhury,Abhishek Sen,Debajyoti Das,Partha Chakrabarti

Cell death discovery 10:365 PubMed39143074

2024

Regulation of Hippo/YAP axis in colon cancer progression by the deubiquitinase JOSD1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Sun,Dongyi Liu,Xiaobo Zhang,Peng Su,Xin Li,Zhongbo Li,Yingwen Gai,Jingying Li,Zhiyong Yang,Yinlu Ding,Jian Zhu,Xiaodong Tan

Cell death & disease 13:1053 PubMed36535926

2022

Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Feng Gao,Xiaoying Li,Yu Gan,Shuangze Han,Xinfang Yu,Haidan Liu,Wei Li

Cell death and differentiation 27:55-70 PubMed31043700

2019

JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Wu,Qingyu Luo,Pengfei Zhao,Wan Chang,Yating Wang,Tong Shu,Fang Ding,Bin Li,Zhihua Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JOSD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JOSD1 antibody (AB118221)

Western blot - Anti-JOSD1 antibody (AB118221)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise