Rabbit Polyclonal JP-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human JPH2.
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- Frontiers in endocrinology 13:8012602022Identification of Crucial Genes and Key Functions in Type 2 Diabetic Hearts by Bioinformatic Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Huang,Kai-Jie Zhang,Jun-Jie Jiang,Shou-Yin Jiang,Jia-Bin Lin,Yi-Jia LouPubMed 35242109
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human JPH2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BR39
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Junctophilin-2. Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes (PubMed:20095964). Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads (By similarity). Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release (By similarity). Contributes to the construction of skeletal muscle triad junctions (By similarity). Junctophilin-2 N-terminal fragment. Transcription repressor required to safeguard against the deleterious effects of cardiac stress. Generated following cleavage of the Junctophilin-2 chain by calpain in response to cardiac stress in cardiomyocytes. Following cleavage and release from the membrane, translocates to the nucleus, binds DNA and represses expression of genes implicated in cell growth and differentiation, hypertrophy, inflammation and fibrosis. Modifies the transcription profile and thereby attenuates pathological remodeling in response to cardiac stress. Probably acts by competing with MEF2 transcription factors and TATA-binding proteins.
Alternative names
JP2, JPH2, Junctophilin-2, JP-2, Junctophilin type 2
Recommended products
Rabbit Polyclonal JP-2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human JPH2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human JPH2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BR39
- Purification technique
- Affinity purification Immunogen
- Specificity
Multiple isoforms of JPH2 are known to exist.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Junctophilin 2 (JP-2) is a protein that plays an important role in the formation of junctional membrane complexes. It belongs to the junctophilin family and has a mass of approximately 65 kDa. JP-2 is expressed in cardiac and skeletal muscle tissues where it facilitates close connections between the plasma membrane and the sarcoplasmic reticulum. These connections are essential for efficient calcium signaling which is integral to muscle contraction and function.
- Biological function summary
JP-2 contributes to the structural bridge between transverse tubules (T-tubules) and the sarcoplasmic reticulum. It is a critical component of the excitation-contraction coupling machinery in muscle cells. JP-2 works as part of a larger protein complex that includes ryanodine receptors and dihydropyridine receptors. These complexes ensure precise delivery and regulation of calcium ions critical to muscle cell contraction.
- Pathways
Junctophilin 2 is closely linked within the calcium signaling and muscle contraction pathways. It partners with proteins like the ryanodine receptor (RyR) and the L-type calcium channel both of which are indispensable for calcium ion movement across the muscle cell membranes. JP-2's interaction with these pathways highlights its role in maintaining calcium homeostasis required for muscle function.
- Associated diseases and disorders
Junctophilin 2 contributes to conditions such as hypertrophic cardiomyopathy and heart failure. Mutations or dysregulation of JP-2 can disrupt normal calcium signaling and lead to these heart disorders. In the context of hypertrophic cardiomyopathy its disruption affects proteins like the ryanodine receptor indicating its pivotal place in both disease development and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-JP-2 antibody (ab110056)
Lane 1: Western blot - Anti-JP-2 antibody (ab110056) at 1 µg/mL
Lane 2: Western blot - Anti-JP-2 antibody (ab110056) at 2 µg/mL
All lanes: Mouse brain tissue lysate
Predicted band size: 74 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JP-2 antibody (ab110056)
ab110056 at 5 μg/ml staining JP-2 in Formalin-Fixed, Paraffin-Embedded Human Heart by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JP-2 antibody (ab110056)
ab110056 at 5 μg/ml staining JP-2 in Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle by Immunohistochemistry.
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