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AB79063

Anti-JPH3 antibody

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(3 Publications)

Rabbit Polyclonal JPH3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human JPH3.

View Alternative Names

JP3, TNRC22, JPH3, Junctophilin-3, JP-3, Junctophilin type 3, Trinucleotide repeat-containing gene 22 protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-JPH3 antibody (AB79063)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JPH3 antibody (AB79063)

Immunofluorescence of JPH3 in Human Brain cells using ab79063 at 20 ug/ml.

Western blot - Anti-JPH3 antibody (AB79063)
  • WB

Unknown

Western blot - Anti-JPH3 antibody (AB79063)

All lanes:

Western blot - Anti-JPH3 antibody (ab79063) at 1 µg/mL

Lane 1:

Daudi cell lysate at 15 µg

Lane 2:

Daudi cell lysate with blocking peptide at 15 µg

Predicted band size: 81 kDa

Observed band size: 100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human JPH3. The exact immunogen used to generate this antibody is proprietary information.

Q8WXH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity chromatography purified via peptide column
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JPH3 also known as junctophilin-3 is a protein that plays a critical role in forming junctional membrane complexes between plasma membrane and endoplasmic or sarcoplasmic reticulum. This protein weighs approximately 82 kDa and helps in maintaining cellular calcium homeostasis. The expression of junctophilin-3 is mainly in the brain particularly within the neuronal tissues indicating its significant role in neural functions.
Biological function summary

Junctophilin-3 contributes to the structural foundation within cells by bridging intracellular membranes with the plasma membrane which is vital for maintaining the proper internal cell architecture. It is a part of a membranous network responsible for calcium ion signaling and plays a role in synaptic function influencing neuronal communication. This function supports the regulation of cellular processes tied to neural activity and development.

Pathways

Junctophilin-3 interacts with key biological pathways such as calcium signaling and neuronal signaling pathways. It connects with these pathways to ensure proper calcium influx necessary for neurotransmitter release and neuronal firing. JPH3 works alongside other proteins like junctophilin-1 in maintaining these pathways highlighting its significance in synaptic transmission and neural excitability.

Alterations in junctophilin-3 expression or function have been associated with neurodegenerative disorders such as Huntington's disease. Research indicates that JPH3 contains a CAG repeat region which when abnormally expanded leads to neuronal dysfunction and degeneration. Furthermore JPH3 interacts with proteins involved in Huntington's disease affecting cellular processes and contributing to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH3 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.
See full target information JPH3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 25: PubMed35169860

2022

Methylation‑associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Zhou Yu,Min Zheng,Xiaohong Yang,Honglan Huang,Lijin Zhao

Cell reports 28:2427-2442.e6 PubMed31461656

2019

Junctophilin Proteins Tether a Cav1-RyR2-KCa3.1 Tripartite Complex to Regulate Neuronal Excitability.

Applications

Unspecified application

Species

Unspecified reactive species

Giriraj Sahu,Rima-Marie Wazen,Pina Colarusso,S R Wayne Chen,Gerald W Zamponi,Ray W Turner

Breast cancer research and treatment 131:75-87 PubMed21340479

2011

Unliganded progesterone receptors attenuate taxane-induced breast cancer cell death by modulating the spindle assembly checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie M Badtke,Purevsuren Jambal,Wendy W Dye,Monique A Spillman,Miriam D Post,Kathryn B Horwitz,Britta M Jacobsen
View all publications
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Product promise

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