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AB254640

Anti-JTB antibody

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Rabbit Polyclonal JTB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human JTB aa 1 to C-terminus.

View Alternative Names

HSPC222, JTB, Protein JTB, Jumping translocation breakpoint protein, Prostate androgen-regulated protein, PAR protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-JTB antibody (AB254640)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JTB antibody (AB254640)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling JTB using ab254640 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JTB antibody (AB254640)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JTB antibody (AB254640)

Paraffin-embedded human stomach tissue stained for JTB using ab254640 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-JTB antibody (AB254640)
  • WB

Supplier Data

Western blot - Anti-JTB antibody (AB254640)

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Western blot - Anti-JTB antibody (ab254640)

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Western blot - Anti-JTB antibody (ab254640) at 0.4 µg/mL

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Control

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JTB overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human JTB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O76095

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JTB also known as Jumping Translocation Breakpoint is a protein with a molecular mass of approximately 14 kDa. It is expressed widely in a variety of tissues with higher levels seen in certain cancers. Mechanically JTB interacts with several cellular structures and its role in the regulation of cell growth and apoptosis has been observed. This protein often localizes to the mitochondria which hints towards its involvement in energy and metabolic processes.
Biological function summary

The JTB protein plays an important role in promoting cell proliferation and survival. It is part of the transmembrane protein complex and is sometimes involved in cellular signaling pathways that manage these processes. Its activity impacts cellular responses to external stress and can contribute to the regulation of cell cycle progression. These roles are consistent with the observed overexpression of JTB in cancerous cells indicating its function in maintaining cell viability.

Pathways

JTB is involved in several signaling pathways that regulate fundamental cellular activities. It is prominently linked with the PI3K/Akt pathway a critical pathway for cell growth and survival. Through this pathway JTB interacts with Akt a significant protein that facilitates cell growth and proliferation. Another connection lies with the MAPK pathway where JTB interacts with key components that influence cell division and differentiation.

Several studies show that JTB has a notable impact on cancer progression particularly in prostate and colorectal cancers. JTB overexpression often correlates with poor prognosis in these cancers aligning with its role in promoting cell proliferation. Additionally JTB's interaction with caspases proteins essential for apoptosis ties it to disorders involving defective apoptosis such as cancer. Understanding these interactions can help target therapies that modulate JTB function and offer new treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1 (By similarity). Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential (By similarity).
See full target information JTB
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Product promise

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