Anti-JUNB Antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-JUNB Antibody (AB327194)

Immunofluorescent staining of human cell line A-431 using ab327194 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUNB Antibody (AB327194)

Immunohistochemical staining of human colon using ab327194 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUNB Antibody (AB327194)

Immunohistochemical staining of human fallopian tube using ab327194 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUNB Antibody (AB327194)

Immunohistochemical staining of human testis using ab327194 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in a subset of cells in seminiferous ducts.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUNB Antibody (AB327194)

Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues using ab327194 at 1 : 200–1 : 500 dilution. Corresponding JUNB RNA-seq data are presented for the same tissues.

• ChIP

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ChIP - Anti-JUNB Antibody (AB327194)

ChIP-Exo-Seq composite graph for ab327194 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-JUNB Antibody (AB327194)

Western blot analysis in human cell lines MCF-7 and HEK293 using ab327194 at 0.04–0.4 µg/ml. Corresponding JUNB RNA-seq data are presented for the same cell lines. Loading control : Anti-PFN1 antibody.

All lanes:

Western blot - Anti-JUNB Antibody (ab327194)

Lane 2:

Human cell lines MCF-7

Lane 3:

Human cell lines HEK293

Predicted band size: 35.88 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUNB Antibody (AB327194)

Immunohistochemical staining of human skeletal muscle using ab327194 at 1 : 200–1 : 500 dilution, showing no positivity in striated muscle fibers as expected.