Rabbit Polyclonal JUNB antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Junb aa 1-100.
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Publications
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- Scientific reports 10:96322020Browning capabilities of human primary adipose-derived stromal cells compared to SGBS cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Halbgebauer et. al.PubMed 32541826
- Journal of dental sciences 15:84-912020Tooth loss, cognitive impairment and chronic cerebral ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Pang,Qianqian Wu,Xingxue Hu,Jianjun Zhang,Qingsong JiangPubMed 32257004
- Frontiers in pharmacology 10:9782019Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3β Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBihao Liu,Jin Lin,Lixia Bai,Yuan Zhou,Ruirui Lu,Peichun Zhang,Dandan Chen,Honglian Li,Jianping Song,Xusheng Liu,Yidan Wu,Junbiao Wu,Chunling Liang,Jiuyao ZhouPubMed 31551783
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Junb aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P09450
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity).
Alternative names
Transcription factor JunB, Transcription factor AP-1 subunit JunB, JUNB
Recommended products
Rabbit Polyclonal JUNB antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Junb aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Junb aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P09450
- Purity
- IgG fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The JunB protein also known as Jun B proto-oncogene is a transcription factor belonging to the AP-1 family. It has a molecular mass of approximately 36 kDa. JunB is expressed in various tissues including epithelial endothelial and immune cells. It often forms heterodimers with other proteins like c-Fos enhancing its capacity to regulate gene expression. Its expression levels can fluctuate with physiological changes and cellular stress indicating its role in managing dynamic cellular processes.
- Biological function summary
JunB influences cell proliferation differentiation and apoptosis. As part of the AP-1 transcription factor complex it binds to specific DNA sequences regulating genes involved in these important processes. This ability to influence cell cycle and immune response highlights the importance of JunB in tissue development and function. Additionally JunB plays a role in controlling the expression of genes involved in inflammatory responses impacting immune cell activation and function.
- Pathways
JunB interacts with various signaling cascades such as the MAPK/ERK and JAK/STAT pathways. These pathways are fundamental in transmitting extracellular signals to the nucleus affecting gene transcription. In the MAPK/ERK pathway JunB works closely with proteins such as c-Jun and c-Fos facilitating cell proliferation and survival. Furthermore it contributes to the JAK/STAT pathway by modulating the inflammatory response demonstrating JunB's adaptability across different cellular contexts.
- Associated diseases and disorders
Altered JunB expression links to conditions like cancer and autoimmune diseases. In some cancers such as leukemia aberrant levels of JunB disrupt normal cell growth collaborating with proteins like c-Myc to promote oncogenesis. In autoimmune disorders JunB can modify immune cell activity through proteins like STAT3 potentially exacerbating inflammation. Understanding JunB functions in these contexts provides insights into potential therapeutic targets for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-JunB antibody (ab3525)
ab3525 in Western blot on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.
All lanes: Western blot - Anti-JunB antibody (ab3525)
Lane 1: L929 cell extract
Lane 2: Extract from L929 cells treated with dexamethasone
Predicted band size: 36 kDa
Observed band size: 38 kDa, 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody (ab3525)
Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling JunB. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3525 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
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