Anti-JunB antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody (AB3525)

Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling JunB. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3525 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

• WB

Unknown

Western blot - Anti-JunB antibody (AB3525)

ab3525 in Western blot on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.

All lanes:

Western blot - Anti-JunB antibody (ab3525)

Lane 1:

L929 cell extract

Lane 2:

Extract from L929 cells treated with dexamethasone

Predicted band size: 36 kDa

Observed band size: 38 kDa,50 kDa

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