JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232033

Anti-JunB antibody [EPR6518] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal JUNB antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Transcription factor JunB, Transcription factor AP-1 subunit JunB, JUNB

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1 : 200 dilution (9.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

Immunoprecipitation - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)
  • IP

Unknown

Immunoprecipitation - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)

ab128878 (purified) at 1 : 100 dilution (2 μg) immunoprecipitating JunB in HeLa whole cell lysates.

Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+) : ab128878 & HeLa whole cell lysates
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878).

All lanes:

Immunoprecipitation - Anti-JunB antibody [EPR6518] (<a href='/en-us/products/primary-antibodies/junb-antibody-epr6518-ab128878'>ab128878</a>)

Predicted band size: 112 kDa,31 kDa,36 kDa,38 kDa,40 kDa,55 kDa

Observed band size: 45 kDa

false

OI-RD Scanning - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-JunB antibody [EPR6518] - BSA and Azide free (AB232033)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6518

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab232033 is the carrier-free version of ab128878.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JunB protein also known as Jun B proto-oncogene is a transcription factor belonging to the AP-1 family. It has a molecular mass of approximately 36 kDa. JunB is expressed in various tissues including epithelial endothelial and immune cells. It often forms heterodimers with other proteins like c-Fos enhancing its capacity to regulate gene expression. Its expression levels can fluctuate with physiological changes and cellular stress indicating its role in managing dynamic cellular processes.
Biological function summary

JunB influences cell proliferation differentiation and apoptosis. As part of the AP-1 transcription factor complex it binds to specific DNA sequences regulating genes involved in these important processes. This ability to influence cell cycle and immune response highlights the importance of JunB in tissue development and function. Additionally JunB plays a role in controlling the expression of genes involved in inflammatory responses impacting immune cell activation and function.

Pathways

JunB interacts with various signaling cascades such as the MAPK/ERK and JAK/STAT pathways. These pathways are fundamental in transmitting extracellular signals to the nucleus affecting gene transcription. In the MAPK/ERK pathway JunB works closely with proteins such as c-Jun and c-Fos facilitating cell proliferation and survival. Furthermore it contributes to the JAK/STAT pathway by modulating the inflammatory response demonstrating JunB's adaptability across different cellular contexts.

Altered JunB expression links to conditions like cancer and autoimmune diseases. In some cancers such as leukemia aberrant levels of JunB disrupt normal cell growth collaborating with proteins like c-Myc to promote oncogenesis. In autoimmune disorders JunB can modify immune cell activity through proteins like STAT3 potentially exacerbating inflammation. Understanding JunB functions in these contexts provides insights into potential therapeutic targets for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity).
See full target information Transcription factor jun-B
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB53543

Anti-JunB antibody [204C4a]

Western blot - Anti-JunB antibody [204C4a] (AB53543)

  • 0
  • 0
Primary Antibodies

AB3525

Anti-JunB antibody

Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody (AB3525)

  • 0
  • 0
Primary Antibodies

AB264302

Anti-JunB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunB antibody (AB264302)

  • 0
  • 0
Primary Antibodies

AB245500

Anti-JunB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunB antibody (AB245500)

  • 0
  • 0
Primary Antibodies

AB200515

Alexa Fluor® 488 Anti-JunB antibody [EPR6518]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-JunB antibody [EPR6518] (AB200515)

  • 0
  • 0
Primary Antibodies

AB200516

Alexa Fluor® 647 Anti-JunB antibody [EPR6518]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-JunB antibody [EPR6518] (AB200516)

  • 5
  • 5

Complementary Products

Primary Antibodies

AB40766

Anti-c-Jun antibody [EP693Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] (AB40766)

  • 5
  • 2
Primary Antibodies

AB181615

Anti-JunD antibody [EPR17365]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (AB181615)

  • 3
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB184938

Anti-Fos B antibody [EPR15905]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Fos B antibody [EPR15905] (AB184938)

  • 5
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com