Rabbit Polyclonal Junctional Adhesion Molecule 1/JAM-A antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
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- Evidence-based complementary and alternative medicine : eCAM 2021:55366792021Sishen Pill Maintained Colonic Mucosal Barrier Integrity to Treat Ulcerative Colitis via Rho/ROCK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Yun Zhang,Hai-Mei Zhao,Yi Liu,Xiu-Yun Lu,Yan-Zhen Li,Qi-Hong Pan,Hai-Yan Wang,Wei Ge,Duan-Yong LiuPubMed 34925530
- Neuro-oncology 23:63-752020NEO100 enables brain delivery of blood‒brain barrier impermeable therapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijun Wang,Nagore I Marín-Ramos,Haiping He,Shan Zeng,Hee-Yeon Cho,Stephen D Swenson,Long Zheng,Alan L Epstein,Axel H Schönthal,Florence M Hofman,Ligang Chen,Thomas C ChenPubMed 32877532
- Journal of applied microbiology 128:1163-11782019Prevention of excessive exercise-induced adverse effects in rats with Bacillus subtilis BSB3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH A G Ducray,L Globa,O Pustovyy,M D Roberts,M Rudisill,V Vodyanoy,I SorokulovaPubMed 31814258
- Journal of applied microbiology 127:1192-12062019Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats.Applications:WBReactive species:RatH A G Ducray,L Globa,O Pustovyy,E Morrison,V Vodyanoy,I SorokulovaPubMed 31230390
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840). (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.
Alternative names
CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1
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Rabbit Polyclonal Junctional Adhesion Molecule 1/JAM-A antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.
- Biological function summary
JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.
- Pathways
JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.
- Associated diseases and disorders
JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.
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Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886)
Junctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Brain (Rat) Tissue Lysate at 10 µg
Lane 3: Mouse Cortex Tissue Lysate at 10 µg
Lane 4: Rat Cortex Tissue Lysate at 10 µg
Lane 5: Cerebellum Mouse Tissue Lysate at 10 µg
Lane 6: Spinal Cord (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 42 kDa, 53 kDa, 99 kDa
Exposure time: 3min
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