Anti-Junctional Adhesion Molecule 1/JAM-A antibody

• WB

Unknown

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB125886)

Junctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate at 10 µg

Lane 3:

Mouse Cortex Tissue Lysate at 10 µg

Lane 4:

Rat Cortex Tissue Lysate at 10 µg

Lane 5:

Cerebellum Mouse Tissue Lysate at 10 µg

Lane 6:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 32 kDa

Observed band size: 42 kDa,53 kDa,99 kDa

true

Exposure time: 3min