AB180821

Anti-Junctional Adhesion Molecule 1/JAM-A antibody

Name: Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab180821) | Abcam
Brand: Abcam Limited
SKU: AB180821
Price: 585 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(19 Publications)

Rabbit Polyclonal Junctional Adhesion Molecule 1/JAM-A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F11R aa 1 to C-terminus.

View Alternative Names

CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB180821)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB180821)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180821. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB180821)

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab180821) at 1/500 dilution

All lanes:

HeLa cell lysate

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human F11R aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y624

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.

Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed : 11812992). Involved in platelet activation (PubMed : 10753840).. (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

See full target information F11R

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

BMC microbiology 24:96 PubMed38521930

2024

Human-derived bacterial strains mitigate colitis via modulating gut microbiota and repairing intestinal barrier function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Dai,Mingjie Jiang,Xiaoxin Wang,Tao Lang,Leilei Wan,Jingjing Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306297 PubMed38477534

2024

Gastrointestinal Dysmotility Predisposes to Colitis through Regulation of Gut Microbial Composition and Linoleic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Youhua Zhang,Feifei Song,Muqing Yang,Chunqiu Chen,Jiaqu Cui,Mengyu Xing,Yuna Dai,Man Li,Yuan Cao,Ling Lu,Huiyuan Zhu,Ying Liu,Chunlian Ma,Qing Wei,Huanlong Qin,Jiyu Li

PloS one 18:e0287597 PubMed37428806

2023

MicroRNA-29b-3p promotes intestinal permeability in IBS-D via targeting TRAF3 to regulate the NF-κB-MLCK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfu Wang,Wei Ke,Jianfeng Gan,He Zhu,Xiangyu Xie,Guodong He,Shan Liu,Yusheng Huang,Hongmei Tang

Journal of oral rehabilitation 50:223-233 PubMed36482049

2022

The effect of occlusal disharmony on a chronic stress-induced animal model of gut microbiota dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Sihui Zhang,Mi Zhang,Xudong Wang,Shuxian Li,Xiaohui Rausch-Fan,Jiang Chen

Precision clinical medicine 5:pbac020 PubMed36132055

2022

JAM-A facilitates hair follicle regeneration in alopecia areata through functioning as ceRNA to protect VCAN expression in dermal papilla cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjuan Wu,Chen Xu,Junfeng Jiang,Sha Xu,Jun Xiong,Xiaoming Fan,Kaihong Ji,Yunpeng Zhao,Haitao Ni,Yue Wang,Houqi Liu,Zhaofan Xia

BMC complementary medicine and therapies 22:157 PubMed35698113

2022

The protective effects of hyperoside on Ang II-mediated apoptosis of bEnd.3 cells and injury of blood-brain barrier model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yan Xie,Yun Wei Lu,Gu Ran Yu

PloS one 17:e0267831 PubMed35639684

2022

Lactobacillus plantarum improves LPS-induced Caco2 cell line intestinal barrier damage via cyclic AMP-PKA signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Xiang Wei,Ju-Hua Wu,Yue-Hong Huang,Xiao-Zhong Wang,Jian-Ying Li

Frontiers in pharmacology 12:759027 PubMed35095486

2022

QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Ye,Quan Li,Chun-Shui Pan,Li Yan,Kai Sun,Xiao-Yi Wang,Shu-Qi Yao,Jing-Yu Fan,Jing-Yan Han

Nature cancer 2:545-562 PubMed35122017

2021

Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Sheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F Quail

Frontiers in pharmacology 12:619436 PubMed33815104

2021

Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Huang,Ruilin Zhang,Shangwen Wang,Dongxian Zhang,Chi-Kwan Leung,Genmeng Yang,Yuanyuan Li,Liu Liu,Yue Xu,Shucheng Lin,Chan Wang,Xiaofeng Zeng,Juan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Junctional Adhesion Molecule 1/JAM-A antibody

Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB180821)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (AB180821)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise