AB232338

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 1/JAM-A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (AB232338)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (AB232338)

Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52647).

Supplier Data

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (AB232338)

Lane 1 : Wild-type HAP1 whole cell lysate (40 μg)
Lane 2 : Junctional Adhesion Molecule 1/JAM-A knockout HAP1 whole cell lysate (40 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab52647 observed at 32 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab52647 was shown to specifically react with Junctional Adhesion Molecule 1/JAM-A in wild-type HAP1 cells as signal was lost in Junctional Adhesion Molecule 1 knockout cells. Wild-type and Junctional Adhesion Molecule 1/JAM-A knockout samples were subjected to SDS-PAGE. ab52647 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52647).

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (<a href='/en-us/products/primary-antibodies/junctional-adhesion-molecule-1-jam-a-antibody-ep1042y-ab52647'>ab52647</a>)

Predicted band size: 32 kDa

false

Unconjugated

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1042Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab232338 is the carrier-free version of ab52647.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.

Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed : 11812992). Involved in platelet activation (PubMed : 10753840).. (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

See full target information F11R

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 2:545-562 PubMed35122017

2021

Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Sheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F Quail

Cancer biology & therapy 21:907-914 PubMed32985327

2020

Long non-coding RNA ARAP1-AS1 promotes the proliferation and migration in cervical cancer through epigenetic regulation of DUSP5.

Applications

Unspecified application

Species

Unspecified reactive species

Han Min,Wenjing He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (AB232338)

Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (AB232338)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise