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Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 2/JAM-B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.


Images

Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (AB156586), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (AB156586), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes (PubMed:11590146, PubMed:11823489, PubMed:24357068). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow (PubMed:24357068). At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed:11590146, PubMed:24357068). Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium (PubMed:12239159). Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1 (PubMed:12070135). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons (By similarity). During myogenesis, it is involved in myocyte fusion (By similarity). May also play a role in angiogenesis (By similarity).

Alternative names

CD322, C21orf43, VEJAM, UNQ219/PRO245, JAM2, Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, Vascular endothelial junction-associated molecule, JAM-2, VE-JAM

Recommended products

Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 2/JAM-B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2489(2)
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Junctional Adhesion Molecule 2 also known as JAM-B JAM B or JAM Protein is a member of the immunoglobulin superfamily of proteins. It has a molecular mass of approximately 38 kDa and is expressed on the surface of endothelial and epithelial cells. JAM-B plays an important role in regulating cellular adhesion facilitating the interaction between adjacent cells. This target is important in maintaining cell polarity and integrity making it an adhesion example of the body’s cellular architecture.

Biological function summary

Junctional adhesion molecule 2 participates in a range of processes related to the formation and maintenance of tight junctions. It is a component of the tight junction complex interacting with other proteins to influence barrier function in vascular and epithelial tissues. This molecule is involved in leukocyte transmigration which is essential during inflammation as leukocytes need to exit the bloodstream to reach sites of infection or injury. It partners with other tight junction proteins which assists in its role in cell adhesion dynamics.

Pathways

JAM-B is involved in the regulation of the leukocyte adhesion cascade and tight junction signaling. Its function is interlinked with the integrin and chemokine pathways influencing leukocyte migration. Junctional adhesion molecule 2 associates with proteins like integrins and PECAM-1 playing a part in the orchestration of cellular movements and interactions that are critical to proper immune response and tissue homeostasis.

Associated diseases and disorders

Junctional adhesion molecule 2 correlation exists with inflammatory diseases and cancer. Its role in leukocyte transmigration links it to inflammatory conditions where increased or disrupted JAM-B expression can affect immune cell infiltration and inflammation severity. In cancer aberrant JAM-B expression can influence tumor growth and metastasis due to its impact on cell adhesion and migration. JAM-B interacts with PD-1 in the context of these diseases highlighting its part in immune regulation and signaling networks associated with tumor progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586), expandable thumbnail

    Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586)

    All lanes: Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586) at 1/1000 dilution

    Lane 1: HUVEC cell lysates at 10 µg

    Lane 2: HeLa cell lysates at 10 µg

    Lane 3: JAR cell lysates at 10 µg

    Lane 4: A431 cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586)

    Intracellular flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2/JAM-B using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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