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AB249274

Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Junctional Adhesion Molecule 2/JAM-B antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CD322, C21orf43, VEJAM, UNQ219/PRO245, JAM2, Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, Vascular endothelial junction-associated molecule, JAM-2, VE-JAM

2 Images
Flow Cytometry (Intracellular) - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free (AB249274)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free (AB249274)

This data was developed using ab156586, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2/JAM-B using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).

Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free (AB249274)
  • WB

Unknown

Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free (AB249274)

This data was developed using ab156586, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (<a href='/en-us/products/primary-antibodies/junctional-adhesion-molecule-2-jam-b-antibody-epr24892-ab156586'>ab156586</a>) at 1/1000 dilution

Lane 1:

HUVEC cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

JAR cell lysates at 10 µg

Lane 4:

A431 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

  • Unconjugated

    Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2489(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab249274 is the carrier-free version of ab156586.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 2 also known as JAM-B JAM B or JAM Protein is a member of the immunoglobulin superfamily of proteins. It has a molecular mass of approximately 38 kDa and is expressed on the surface of endothelial and epithelial cells. JAM-B plays an important role in regulating cellular adhesion facilitating the interaction between adjacent cells. This target is important in maintaining cell polarity and integrity making it an adhesion example of the body’s cellular architecture.
Biological function summary

Junctional adhesion molecule 2 participates in a range of processes related to the formation and maintenance of tight junctions. It is a component of the tight junction complex interacting with other proteins to influence barrier function in vascular and epithelial tissues. This molecule is involved in leukocyte transmigration which is essential during inflammation as leukocytes need to exit the bloodstream to reach sites of infection or injury. It partners with other tight junction proteins which assists in its role in cell adhesion dynamics.

Pathways

JAM-B is involved in the regulation of the leukocyte adhesion cascade and tight junction signaling. Its function is interlinked with the integrin and chemokine pathways influencing leukocyte migration. Junctional adhesion molecule 2 associates with proteins like integrins and PECAM-1 playing a part in the orchestration of cellular movements and interactions that are critical to proper immune response and tissue homeostasis.

Junctional adhesion molecule 2 correlation exists with inflammatory diseases and cancer. Its role in leukocyte transmigration links it to inflammatory conditions where increased or disrupted JAM-B expression can affect immune cell infiltration and inflammation severity. In cancer aberrant JAM-B expression can influence tumor growth and metastasis due to its impact on cell adhesion and migration. JAM-B interacts with PD-1 in the context of these diseases highlighting its part in immune regulation and signaling networks associated with tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes (PubMed : 11590146, PubMed : 11823489, PubMed : 24357068). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow (PubMed : 24357068). At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed : 11590146, PubMed : 24357068). Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium (PubMed : 12239159). Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1 (PubMed : 12070135). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons (By similarity). During myogenesis, it is involved in myocyte fusion (By similarity). May also play a role in angiogenesis (By similarity).
See full target information JAM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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