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AB327256

Anti-JUND Antibody

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Rabbit Polyclonal JUND antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transcription factor jun-D.

View Alternative Names

Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)

Immunohistochemical staining of human placenta using ab327256 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in trophoblastic cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)

Immunohistochemical staining of human cerebral cortex using ab327256 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in neuronal and glial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JUND Antibody (AB327256)

Immunohistochemical staining of human testis using ab327256 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in cells in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-JUND Antibody (AB327256)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JUND Antibody (AB327256)

Immunofluorescent staining of human cell line SH-SY5Y using ab327256 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-JUND Antibody (AB327256)
  • ChIP

Supplier Data

ChIP - Anti-JUND Antibody (AB327256)

ChIP-Exo-Seq composite graph for ab327256 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ChIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Transcription factor jun-D.

P17535

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor binding AP-1 sites (PubMed : 9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed : 28981703, PubMed : 9989505).
See full target information Transcription factor jun-D
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Product promise

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