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Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511), expandable thumbnail
  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511), expandable thumbnail
  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511), expandable thumbnail
  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Transcription factor binding AP-1 sites.

    Alternative names

    Transcription factor jun-D, JUND

    Recommended products

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    Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.

    Alternative names

    Transcription factor jun-D, JUND

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR17365

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab250511 is the carrier-free version of Anti-JunD antibody [EPR17365] ab181615.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    The JunD transcription factor also known as JunD protein belongs to the AP-1 family of transcription factors. It has a mass of approximately 39 kDa. JunD primarily acts by forming dimers with other proteins such as c-Fos and c-Jun. These dimers bind to specific DNA sequences regulating gene expression. JunD shows expression in various tissues but is more abundant in non-dividing and differentiated cells where it helps modulate specific cellular processes.

    Biological function summary

    JunD plays a significant role by influencing cellular proliferation and differentiation. It is often part of a multiprotein complex which can include members of the Fos family and other Jun proteins. These complexes interact with DNA to control the transcription of genes involved in cell cycle regulation apoptosis and stress responses. Different stimuli can alter the composition of these complexes highlighting JunD's adaptability in cellular signaling.

    Pathways

    JunD interacts within the MAPK signaling pathway and the oxidative stress response pathway. In the MAPK pathway it can interact with proteins such as ERK and JNK which are critical mediators of cellular responses to growth factors and stress. In the oxidative stress response JunD regulates genes that protect cells from oxidative damage showing its influence on maintaining cellular homeostasis. Other proteins like ATF2 may also interact with JunD within these pathways contributing to transcriptional regulation.

    Associated diseases and disorders

    JunD has connections to cancer and neurodegenerative diseases. Its role in regulating cell proliferation links it to tumorigenesis where alterations in its function can lead to uncontrolled cell growth. Additionally in neurodegenerative disorders JunD's influence on oxidative stress response genes may impact disease progression. Furthermore JunD's interaction with the protein Bcl2 associates it with the protection of cells from apoptotic signals illustrating its potential impact on disease development and progression.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    9 product images

    • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      All lanes: Western blot - Anti-JunD antibody [EPR17365] (Anti-JunD antibody [EPR17365] ab181615) at 1/1000 dilution

      Lane 1: 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

      Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

      Lane 3: Human fetal liver lysate at 10 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 35 kDa

      Observed band size: 39 kDa, 42 kDa

      Exposure time: 3min

    • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      All lanes: Western blot - Anti-JunD antibody [EPR17365] (Anti-JunD antibody [EPR17365] ab181615) at 1/1000 dilution

      All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 35 kDa

      Observed band size: 39 kDa, 42 kDa

      Exposure time: 5s

    • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      All lanes: Western blot - Anti-JunD antibody [EPR17365] (Anti-JunD antibody [EPR17365] ab181615) at 1/1000 dilution

      Lane 1: Human fetal brain lysate at 10 µg

      Lane 2: Human fetal heart lysate at 10 µg

      Lane 3: Human fetal kidney lysate at 10 µg

      Secondary

      All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 35 kDa

      Observed band size: 39 kDa, 42 kDa

      Exposure time: 1min

    • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      All lanes: Western blot - Anti-JunD antibody [EPR17365] (Anti-JunD antibody [EPR17365] ab181615) at 1/1000 dilution

      Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

      Lane 2: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg

      Lane 3: PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 10 µg

      Lane 4: NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate at 10 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 35 kDa

      Observed band size: 39 kDa, 42 kDa

      Exposure time: 30s

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using Anti-JunD antibody [EPR17365] ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of Anti-JunD antibody [EPR17365] ab181615 and secondary antibody only.
      Note: Nuclear staining on the epithelial cells of Human mammary gland was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using Anti-JunD antibody [EPR17365] ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of Anti-JunD antibody [EPR17365] ab181615 and secondary antibody only.
      Note: Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using Anti-JunD antibody [EPR17365] ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of Anti-JunD antibody [EPR17365] ab181615 and secondary antibody only.
      Note: Nuclear staining on neurons of the mouse cerebral cortex was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using Anti-JunD antibody [EPR17365] ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
      Inset image: negative control obtained using PBS instead of Anti-JunD antibody [EPR17365] ab181615 and secondary antibody only.
      Note: Nuclear staining on neurons of the rat cerebral cortex was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

      This data was developed using Anti-JunD antibody [EPR17365] ab181615, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with Anti-JunD antibody [EPR17365] ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      1. Anti-JunD antibody [EPR17365] ab181615 at 1/10000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

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