JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250511

Anti-JunD antibody [EPR17365] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on the epithelial cells of Human mammary gland was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on neurons of the rat cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note : Nuclear staining on neurons of the mouse cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (<a href='/en-us/products/primary-antibodies/jund-antibody-epr17365-ab181615'>ab181615</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 1min

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (<a href='/en-us/products/primary-antibodies/jund-antibody-epr17365-ab181615'>ab181615</a>) at 1/1000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 5s

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (<a href='/en-us/products/primary-antibodies/jund-antibody-epr17365-ab181615'>ab181615</a>) at 1/1000 dilution

Lane 1:

293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 3min

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)
  • WB

Supplier Data

Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (AB250511)

This data was developed using ab181615, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JunD antibody [EPR17365] (<a href='/en-us/products/primary-antibodies/jund-antibody-epr17365-ab181615'>ab181615</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate at 10 µg

Lane 3:

PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,42 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17365

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB128878

Anti-JunB antibody [EPR6518]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-JunB antibody [EPR6518] (AB128878)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB40766

Anti-c-Jun antibody [EP693Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] (AB40766)

  • 5
  • 2
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250511 is the carrier-free version of ab181615.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JunD transcription factor also known as JunD protein belongs to the AP-1 family of transcription factors. It has a mass of approximately 39 kDa. JunD primarily acts by forming dimers with other proteins such as c-Fos and c-Jun. These dimers bind to specific DNA sequences regulating gene expression. JunD shows expression in various tissues but is more abundant in non-dividing and differentiated cells where it helps modulate specific cellular processes.
Biological function summary

JunD plays a significant role by influencing cellular proliferation and differentiation. It is often part of a multiprotein complex which can include members of the Fos family and other Jun proteins. These complexes interact with DNA to control the transcription of genes involved in cell cycle regulation apoptosis and stress responses. Different stimuli can alter the composition of these complexes highlighting JunD's adaptability in cellular signaling.

Pathways

JunD interacts within the MAPK signaling pathway and the oxidative stress response pathway. In the MAPK pathway it can interact with proteins such as ERK and JNK which are critical mediators of cellular responses to growth factors and stress. In the oxidative stress response JunD regulates genes that protect cells from oxidative damage showing its influence on maintaining cellular homeostasis. Other proteins like ATF2 may also interact with JunD within these pathways contributing to transcriptional regulation.

JunD has connections to cancer and neurodegenerative diseases. Its role in regulating cell proliferation links it to tumorigenesis where alterations in its function can lead to uncontrolled cell growth. Additionally in neurodegenerative disorders JunD's influence on oxidative stress response genes may impact disease progression. Furthermore JunD's interaction with the protein Bcl2 associates it with the protection of cells from apoptotic signals illustrating its potential impact on disease development and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor binding AP-1 sites (PubMed : 9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed : 28981703, PubMed : 9989505).
See full target information Transcription factor jun-D
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com