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AB134067

Anti-JunD antibody [EPR6520]

5

(2 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal JUND antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)

Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200 counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

Western blot - Anti-JunD antibody [EPR6520] (AB134067)
  • WB

Unknown

Western blot - Anti-JunD antibody [EPR6520] (AB134067)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,43 kDa

false

Western blot - Anti-JunD antibody [EPR6520] (AB134067)
  • WB

Unknown

Western blot - Anti-JunD antibody [EPR6520] (AB134067)

All lanes:

Western blot - Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa,43 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)

Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300 counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] (AB134067)

Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)
  • IP

Lab

Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)

ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)

Predicted band size: 35 kDa

false

Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)
  • IP

Lab

Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)

ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-JunD antibody [EPR6520] (ab134067)

Predicted band size: 35 kDa

false

OI-RD Scanning - Anti-JunD antibody [EPR6520] (AB134067)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-JunD antibody [EPR6520] (AB134067)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6520

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p><strong>For unpurified, use 1/30.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p><strong>For unpurified, use 1/5000.</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "<p><strong>For unpurified, use 1/200.</strong></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JunD transcription factor also known as JunD protein belongs to the AP-1 family of transcription factors. It has a mass of approximately 39 kDa. JunD primarily acts by forming dimers with other proteins such as c-Fos and c-Jun. These dimers bind to specific DNA sequences regulating gene expression. JunD shows expression in various tissues but is more abundant in non-dividing and differentiated cells where it helps modulate specific cellular processes.
Biological function summary

JunD plays a significant role by influencing cellular proliferation and differentiation. It is often part of a multiprotein complex which can include members of the Fos family and other Jun proteins. These complexes interact with DNA to control the transcription of genes involved in cell cycle regulation apoptosis and stress responses. Different stimuli can alter the composition of these complexes highlighting JunD's adaptability in cellular signaling.

Pathways

JunD interacts within the MAPK signaling pathway and the oxidative stress response pathway. In the MAPK pathway it can interact with proteins such as ERK and JNK which are critical mediators of cellular responses to growth factors and stress. In the oxidative stress response JunD regulates genes that protect cells from oxidative damage showing its influence on maintaining cellular homeostasis. Other proteins like ATF2 may also interact with JunD within these pathways contributing to transcriptional regulation.

JunD has connections to cancer and neurodegenerative diseases. Its role in regulating cell proliferation links it to tumorigenesis where alterations in its function can lead to uncontrolled cell growth. Additionally in neurodegenerative disorders JunD's influence on oxidative stress response genes may impact disease progression. Furthermore JunD's interaction with the protein Bcl2 associates it with the protection of cells from apoptotic signals illustrating its potential impact on disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor binding AP-1 sites (PubMed : 9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed : 28981703, PubMed : 9989505).
See full target information Transcription factor jun-D
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 13:170-186 PubMed36908856

2023

SUMO1 regulates post-infarct cardiac repair based on cellular heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Liu,Xiaozhi Liu,Li Liu,Ying Wang,Jie Zheng,Lan Li,Sheng Li,Han Zhang,Jingyu Ni,Chuanrui Ma,Xiumei Gao,Xiyun Bian,Guanwei Fan

Theranostics 12:3928-3945 PubMed35664068

2022

5-HT2B-mediated serotonin activation in enterocytes suppresses colitis-associated cancer initiation and promotes cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Mao,Fang Xin,Jie Ren,Shuai Xu,Haixia Huang,Xu Zha,Xinxin Wen,Guoqing Gu,Guang Yang,Yuan Cheng,Chen Zhang,Wei Wang,Xicheng Liu

Rheumatology (Oxford, England) 61:3427-3438 PubMed34849618

2021

Chondroprotective effects of CDK4/6 inhibition via enhanced ubiquitin-dependent degradation of JUN in synovial fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Tadashi Hosoya,Tetsuya Saito,Hiroyuki Baba,Nao Tanaka,Seiji Noda,Youji Komiya,Yasuhiro Tagawa,Akio Yamamoto,Fumitaka Mizoguchi,Kimito Kawahata,Nobuyuki Miyasaka,Hitoshi Kohsaka,Shinsuke Yasuda

JCI insight 4: PubMed31578304

2019

The antioxidant N-acetylcysteine protects from lung emphysema but induces lung adenocarcinoma in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marielle Breau,Amal Houssaini,Larissa Lipskaia,Shariq Abid,Emmanuelle Born,Elisabeth Marcos,Gabor Czibik,Aya Attwe,Delphine Beaulieu,Alberta Palazzo,Jean-Michel Flaman,Brigitte Bourachot,Guillaume Collin,Jeanne Tran Van Nhieu,David Bernard,Fatima Mechta-Grigoriou,Serge Adnot

The international journal of biochemistry & cell b 104:73-86 PubMed30236993

2018

A MAPK/c-Jun-mediated switch regulates the initial adaptive and cell death responses to mitochondrial damage in a neuronal cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Ryan,Katherine M Roper,Jacquelyn Bond,Sandra M Bell,Sean T Sweeney,Ewan E Morrison

Nature communications 9:3149 PubMed30089879

2018

Transcriptional and physiological adaptations in nucleus accumbens somatostatin interneurons that regulate behavioral responses to cocaine.

Applications

Unspecified application

Species

Unspecified reactive species

Efrain A Ribeiro,Marine Salery,Joseph R Scarpa,Erin S Calipari,Peter J Hamilton,Stacy M Ku,Hope Kronman,Immanuel Purushothaman,Barbara Juarez,Mitra Heshmati,Marie Doyle,Casey Lardner,Dominicka Burek,Ana Strat,Stephen Pirpinias,Ezekiell Mouzon,Ming-Hu Han,Rachael L Neve,Rosemary C Bagot,Andrew Kasarskis,Ja Wook Koo,Eric J Nestler

Nature communications 7:12098 PubMed27384883

2016

Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41.

Applications

EMSA

Species

Unspecified reactive species

Pierre Bigot,Leandro M Colli,Mitchell J Machiela,Lea Jessop,Timothy A Myers,Julie Carrouget,Sarah Wagner,David Roberson,Caroline Eymerit,Daniel Henrion,Stephen J Chanock

Molecular cancer research : MCR 11:1542-53 PubMed24107296

2013

S100A14: novel modulator of terminal differentiation in esophageal cancer.

Applications

WB

Species

Human

Hongyan Chen,Jianlin Ma,Benjamin Sunkel,Aiping Luo,Fang Ding,Yi Li,Huan He,Shuguang Zhang,Chengshan Xu,Qinge Jin,Qianben Wang,Zhihua Liu
View all publications
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Product promise

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