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Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Target data

Function

Transcription factor binding AP-1 sites.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR6520

Purification technique

Affinity purification Protein A

Dissociation constant

1.6 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248667 is the carrier-free version of ab134067.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)

    Predicted band size: 35 kDa

  • Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-JunD antibody [EPR6520] (AB134067) at 1/10000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 35 kDa

    Observed band size: 39 kDa, 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

  • Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)

    Predicted band size: 35 kDa

  • Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-JunD antibody [EPR6520] (AB134067) at 1/10000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa

    Observed band size: 39 kDa, 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

  • OI-RD Scanning - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667), expandable thumbnail

    OI-RD Scanning - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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