Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)

Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (AB248667), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P
Human	Tested	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Target data

See full target information Transcription factor jun-D

Function

Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:28981703, PubMed:9989505).

Alternative names

Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND

Recommended products

Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR6520
Purification technique: Affinity purification Protein A
Dissociation constant: 1.6 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab248667 is the carrier-free version of Anti-JunD antibody [EPR6520] ab134067.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The JunD transcription factor also known as JunD protein belongs to the AP-1 family of transcription factors. It has a mass of approximately 39 kDa. JunD primarily acts by forming dimers with other proteins such as c-Fos and c-Jun. These dimers bind to specific DNA sequences regulating gene expression. JunD shows expression in various tissues but is more abundant in non-dividing and differentiated cells where it helps modulate specific cellular processes.

Biological function summary

JunD plays a significant role by influencing cellular proliferation and differentiation. It is often part of a multiprotein complex which can include members of the Fos family and other Jun proteins. These complexes interact with DNA to control the transcription of genes involved in cell cycle regulation apoptosis and stress responses. Different stimuli can alter the composition of these complexes highlighting JunD's adaptability in cellular signaling.

Pathways

JunD interacts within the MAPK signaling pathway and the oxidative stress response pathway. In the MAPK pathway it can interact with proteins such as ERK and JNK which are critical mediators of cellular responses to growth factors and stress. In the oxidative stress response JunD regulates genes that protect cells from oxidative damage showing its influence on maintaining cellular homeostasis. Other proteins like ATF2 may also interact with JunD within these pathways contributing to transcriptional regulation.

Associated diseases and disorders

JunD has connections to cancer and neurodegenerative diseases. Its role in regulating cell proliferation links it to tumorigenesis where alterations in its function can lead to uncontrolled cell growth. Additionally in neurodegenerative disorders JunD's influence on oxidative stress response genes may impact disease progression. Furthermore JunD's interaction with the protein Bcl2 associates it with the protection of cells from apoptotic signals illustrating its potential impact on disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.Anti-JunD antibody [EPR6520] ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (Anti-JunD antibody [EPR6520] ab134067)
Predicted band size: 35 kDa
Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-JunD antibody [EPR6520] (Anti-JunD antibody [EPR6520] ab134067) at 1/10000 dilution
All lanes: HeLa cell lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa, 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified Anti-JunD antibody [EPR6520] ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified Anti-JunD antibody [EPR6520] ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor^® 488 goat anti-mouse antibody at a dilution of 1/500.
Immunoprecipitation - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.Anti-JunD antibody [EPR6520] ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (Anti-JunD antibody [EPR6520] ab134067)
Predicted band size: 35 kDa
Western blot - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-JunD antibody [EPR6520] (Anti-JunD antibody [EPR6520] ab134067) at 1/10000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa, 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified Anti-JunD antibody [EPR6520] ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified Anti-JunD antibody [EPR6520] ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor^® 488 goat anti-mouse antibody at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
This data was developed using Anti-JunD antibody [EPR6520] ab134067, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling JunD with unpurified Anti-JunD antibody [EPR6520] ab134067 at 1/100 dilution.
OI-RD Scanning - Anti-JunD antibody [EPR6520] - BSA and Azide free (ab248667)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.