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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ICC/IF | IP | WB | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Transcription factor jun-D, JUND
Rabbit Recombinant Monoclonal JUND antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Transcription factor jun-D, JUND
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
Yes
EPR6520
Affinity purification Protein A
1.6 x 10-11 M
Blue Ice
+4°C
Do Not Freeze
ab248667 is the carrier-free version of ab134067.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using ab134067, the same antibody clone in a different buffer formulation.ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)
Predicted band size: 35 kDa
This data was developed using ab134067, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-JunD antibody [EPR6520] (AB134067) at 1/10000 dilution
All lanes: HeLa cell lysate at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa, 43 kDa
This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.
This data was developed using ab134067, the same antibody clone in a different buffer formulation.ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-JunD antibody [EPR6520] (AB134067)
Predicted band size: 35 kDa
This data was developed using ab134067, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-JunD antibody [EPR6520] (AB134067) at 1/10000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 39 kDa, 43 kDa
This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.
This data was developed using ab134067, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com