Anti-JunD Antibody [RP23040103]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JunD Antibody [RP23040103] (AB308053)

Immunofluorescent analysis of 4% paraformaldehyde on 70% confluent log phase HeLa cells  labeling JunD with ab308053 at 5 µg/mL, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/400 (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.

• WB

Supplier Data

Western blot - Anti-JunD Antibody [RP23040103] (AB308053)

Western blot analysis of JunD was performed by loading 50 µg of HeLa (Lane1), A549 (Lane2), Panc1 (Lane 3), HepG2 (Lane 4), K562 (Lane5), Caco2 (Lane6) cell lysates using a 4-12 % Bis-Tris gel electrophoresis system and pre-stained protein standard. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk 1 hour at room temperature on a rocking platform. . JunD was detected at ~42 kDa using ab308053 at 2-3 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody at 1 : 5000 dilution was used and chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-JunD Antibody [RP23040103] (ab308053) at 2 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

A549 cell lysate at 50 µg

Lane 3:

Panc1 cell lysate at 50 µg

Lane 4:

HepG2 cell lysate at 50 µg

Lane 5:

K562 cell lysate at 50 µg

Lane 6:

Caco2 cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG Secondary Antibody at 1/5000 dilution

Observed band size: 42 kDa

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