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AB326637

Anti-JunD (phospho S255) antibody [JJ08-21]

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Rabbit Recombinant Monoclonal JUND phospho S255 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.

View Alternative Names

Transcription factor JunD, Transcription factor AP-1 subunit JunD, JUND

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S255) antibody [JJ08-21] (AB326637)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S255) antibody [JJ08-21] (AB326637)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for JunD (phospho S255) using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-JunD (phospho S255) antibody [JJ08-21] (AB326637)
  • WB

Supplier Data

Western blot - Anti-JunD (phospho S255) antibody [JJ08-21] (AB326637)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates treated with λpp for 1 hour
Lane 2 : Hela whole cell lysates
Lane 3 : PC12 whole cell lysates
Lane 4 : NIH/3T3 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 38 kDa
Observed band size : 42 kDa

Exposure time : 12 seconds

All lanes:

Western blot - Anti-JunD (phospho S255) antibody [JJ08-21] (ab326637) at 1/1000 dilution

Lane 1:

Hela whole cell lysates treated with λpp for 1 hour at 20 µg

Lane 2:

Hela whole cell lysates at 20 µg

Lane 3:

PC12 whole cell lysates at 20 µg

Lane 4:

NIH/3T3 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 42 kDa

false

Exposure time: 12s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

JJ08-21

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor binding AP-1 sites (PubMed : 9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed : 28981703, PubMed : 9989505).
See full target information JUND pS255
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com