AB133632

Anti-K Cadherin/CDH6 antibody [EPR7394]

Name: Anti-K Cadherin/CDH6 antibody [EPR7394] (ab133632) | Abcam
Brand: Abcam Limited
SKU: AB133632
Price: 270 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal K Cadherin/CDH6 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Cadherin-6, Kidney cadherin, K-cadherin, CDH6

2 Images

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Western blot - Anti-K Cadherin/CDH6 antibody [EPR7394] (AB133632)

All lanes:

Western blot - Anti-K Cadherin/CDH6 antibody [EPR7394] (ab133632) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Lane 3:

Fetal brain lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Lane 6:

293T cell lysate at 10 µg

Lane 7:

Fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 88 kDa

Observed band size: 130 kDa

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OI-RD Scanning - Anti-K Cadherin/CDH6 antibody [EPR7394] (AB133632)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-K Cadherin/CDH6 antibody [EPR7394] (AB133632)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-K Cadherin/CDH6 antibody [EPR7394] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7394

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares very high homology with family members Cadherin-9, Cadherin-2, Cadherin-4, Cadherin-7, Cadherin-8, Cadherin-11. Cross-reactivity with these proteins has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K Cadherin also known as CDH6 K-Cadherin or Cadherin-6 is a protein that plays a significant mechanical role in cell adhesion. It belongs to the cadherin superfamily of proteins which are known for their role in calcium-dependent cell-cell adhesion. The CDH6 protein has an approximate molecular mass of 88 kDa. It is mainly expressed in the kidney brain and pancreas where it contributes to maintaining proper cellular architecture and signaling.

Biological function summary

Cadherin-6 is involved in the dynamic regulation of tissue morphogenesis and neural development. It interacts with intracellular catenins to form a cadherin-catenin complex which links to the actin cytoskeleton. This complex is essential for transmitting mechanical signals and maintaining cellular structure. CDH6 plays a critical role in branching morphogenesis and neuronal connectivity aiding in the formation of organized tissue architecture and neural networks.

Pathways

Cadherin-6 is a component of the Wnt signaling pathway which influences cell fate determination and patterning. CDH6 interacts with related proteins like beta-catenin facilitating the transduction of signals within the Wnt pathway. Additionally it is part of the epithelial-mesenchymal transition (EMT) pathway where it influences cell migration and differentiation by interacting with growth factor receptors and downstream signaling molecules.

Dysregulation of cadherin-6 expression links to various cancers such as renal cell carcinoma and glioblastoma. In these contexts altered CDH6 levels can impact tumor progression and metastatic potential. Furthermore its interplay with proteins like E-cadherin and integrins affects cellular proliferation and invasion highlighting the importance of CDH6 in cancer biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH6

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:711 PubMed39487512

2024

Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Zhang,Yanhui Peng,Ting Guo,Wei Fang,Zhengqiang Li,Xiaoqin Yang

Communications biology 3:574 PubMed33060832

2020

Redundant type II cadherins define neuroepithelial cell states for cytoarchitectonic robustness.

Applications

Unspecified application

Species

Unspecified reactive species

Kou Hiraga,Yukiko U Inoue,Junko Asami,Mayuko Hotta,Yuki Morimoto,Shoji Tatsumoto,Mikio Hoshino,Yasuhiro Go,Takayoshi Inoue

Molecular therapy : the journal of the American So 28:2458-2472 PubMed32592690

2020

Tryptophanyl-tRNA Synthetase, a Novel Damage-Induced Cytokine, Significantly Increases the Therapeutic Effects of Endometrial Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Se-Ra Park,Soo-Rim Kim,Jae-Been Im,Soyi Lim,In-Sun Hong

Nature communications 11:1680 PubMed32245949

2020

β-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sining Leng,Emanuele Pignatti,Radhika S Khetani,Manasvi S Shah,Simiao Xu,Ji Miao,Makoto M Taketo,Felix Beuschlein,Paula Q Barrett,Diana L Carlone,David T Breault

Nature communications 11:1679 PubMed32245948

2020

Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes.

Applications

Unspecified application

Species

Unspecified reactive species

Nick A Guagliardo,Peter M Klein,Christina A Gancayco,Adam Lu,Sining Leng,Rany R Makarem,Chelsea Cho,Craig G Rusin,David T Breault,Paula Q Barrett,Mark P Beenhakker

Oncotarget 8:87848-87859 PubMed29152125

2017

CD49b inhibits osteogenic differentiation and plays an important role in osteosarcoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Ren,Sajida Piperdi,Pratistha Koirala,Amy Park,Wendong Zhang,Daria Ivenitsky,Yidan Zhang,Esperanza Villanueva-Siles,Douglas S Hawkins,Michael Roth,Richard Gorlick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-K Cadherin/CDH6 antibody [EPR7394]

Western blot - Anti-K Cadherin/CDH6 antibody [EPR7394] (AB133632)

OI-RD Scanning - Anti-K Cadherin/CDH6 antibody [EPR7394] (AB133632)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise