AB156569

Anti-K23 antibody [EPR10943]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal K23 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Cytokeratin-23, Keratin-23, CK-23, K23, KRT23

3 Images

Flow Cytometry (Intracellular) - Anti-K23 antibody [EPR10943] (AB156569)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-K23 antibody [EPR10943] (AB156569)

Intracellular flow cytometrical analysis of permeabilized JAR cells with ab156569 antibody at a dilution of 1/10 (red) compared to a rabbit IgG (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K23 antibody [EPR10943] (AB156569)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K23 antibody [EPR10943] (AB156569)

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling K23 with ab156569 antibody at 1/150.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-K23 antibody [EPR10943] (AB156569)

All lanes:

Western blot - Anti-K23 antibody [EPR10943] (ab156569) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Carrier free

Anti-K23 antibody [EPR10943] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10943

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K23 also known as SUMO-3 is a small ubiquitin-like modifier protein with a mass of approximately 11 kDa. It shares structural similarities with ubiquitin allowing it to form covalent attachments to target proteins. K23 is ubiquitously expressed in the cell primarily in the nucleus but also found in the cytoplasm. The protein is involved in various cellular processes through reversible post-translational modifications.

Biological function summary

The attachment of K23 to target proteins influences their stability localization and interactions. It often modifies transcription factors and other nuclear proteins altering gene expression patterns. K23 is part of the SUMO modification complex that includes enzymes like E1-activating E2-conjugating and E3-ligase proteins. This post-translational modification process affects several cellular pathways and maintains cellular homeostasis.

Pathways

SUMOylation influences important pathways like DNA damage response and cell cycle regulation. K23 plays a significant role in DNA repair mechanisms and mitotic progression by modifying proteins such as p53 and RanGAP1. These modifications are vital for the proper execution of cellular tasks and communication with related proteins that share these pathways including SUMO-1 and SUMO-2 which often compensate for each other's functions.

Dysfunction in K23 modification contributes to conditions like cancer and neurodegenerative diseases. In cancers abnormal SUMOylation mediated by K23 impacts cellular proliferation and survival pathways often involving dysregulation of proteins like p53. Neurodegenerative diseases also show altered levels of SUMOylated proteins implicating K23 in modifications that affect neuronal function and integrity. Researchers focus on these diseases to explore therapeutic potential targeting the SUMOylation pathway.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Keratin, type I cytoskeletal 23

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 16: PubMed39771533

2025

miR-195-5p Inhibits Colon Cancer Progression via KRT23 Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Piccinno,Viviana Scalavino,Nicoletta Labarile,Raffaele Armentano,Gianluigi Giannelli,Grazia Serino

BMC cancer 24:1024 PubMed39160525

2024

Immune checkpoint gene signature assesses immune infiltration profiles in bladder cancer and identifies KRT23 as an immunotherapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Dongshan Chen,Haoyuan Cao,Xiang Zheng,Haojun Wang,Zengchi Han,Wei Wang

Clinical & translational immunology 13:e1495 PubMed38433762

2024

High KRT17 expression in tumour budding indicates immunologically 'hot' tumour budding and predicts good survival in patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfeng Liang,Haiqing Jie,Hao Xie,Yebohao Zhou,Wenxin Li,Liang Huang,Zhenxing Liang,Huashan Liu,Xiaobin Zheng,Ziwei Zeng,Liang Kang

Molecular medicine reports 29: PubMed38099343

2023

Loss of keratin 23 enhances growth inhibitory effect of melatonin in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Meifang Lin,Jianhua Luo,Huaqin Sun,Zhiguang Zhang,Dacen Lin,Lushan Chen,Sisi Feng,Xiuping Lin,Ruixiang Zhou,Jun Song

Cell systems 13:644-664.e8 PubMed35863345

2022

Mapping hormone-regulated cell-cell interaction networks in the human breast at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Lyndsay M Murrow,Robert J Weber,Joseph A Caruso,Christopher S McGinnis,Kiet Phong,Philippe Gascard,Gabrielle Rabadam,Alexander D Borowsky,Tejal A Desai,Matthew Thomson,Thea Tlsty,Zev J Gartner

Cells 8: PubMed31216713

2019

Identification of Keratin 23 as a Hepatitis C Virus-Induced Host Factor in the Human Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Volker Kinast,Stefan L Leber,Richard J P Brown,Gabrielle Vieyres,Patrick Behrendt,Constanze Eßbach,Pavel Strnad,Florian W R Vondran,Markus Cornberg,Cora Wex,Thomas Pietschmann,Johannes Haybaeck,Daniel Todt,Eike Steinmann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-K23 antibody [EPR10943]

Flow Cytometry (Intracellular) - Anti-K23 antibody [EPR10943] (AB156569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K23 antibody [EPR10943] (AB156569)

Western blot - Anti-K23 antibody [EPR10943] (AB156569)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise