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AB249264

Anti-K23 antibody [EPR10943] - BSA and Azide free

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Rabbit Recombinant Monoclonal K23 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Cytokeratin-23, Keratin-23, CK-23, K23, KRT23

3 Images
Flow Cytometry (Intracellular) - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)

This data was developed using ab156569, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized JAR cells with ab156569 antibody at a dilution of 1/10 (red) compared to a rabbit IgG (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)

This data was developed using ab156569, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling K23 with ab156569 antibody at 1/150.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)
  • WB

Unknown

Western blot - Anti-K23 antibody [EPR10943] - BSA and Azide free (AB249264)

This data was developed using ab156569, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-K23 antibody [EPR10943] (<a href='/en-us/products/primary-antibodies/k23-antibody-epr10943-ab156569'>ab156569</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10943

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249264 is the carrier-free version of ab156569.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K23 also known as SUMO-3 is a small ubiquitin-like modifier protein with a mass of approximately 11 kDa. It shares structural similarities with ubiquitin allowing it to form covalent attachments to target proteins. K23 is ubiquitously expressed in the cell primarily in the nucleus but also found in the cytoplasm. The protein is involved in various cellular processes through reversible post-translational modifications.
Biological function summary

The attachment of K23 to target proteins influences their stability localization and interactions. It often modifies transcription factors and other nuclear proteins altering gene expression patterns. K23 is part of the SUMO modification complex that includes enzymes like E1-activating E2-conjugating and E3-ligase proteins. This post-translational modification process affects several cellular pathways and maintains cellular homeostasis.

Pathways

SUMOylation influences important pathways like DNA damage response and cell cycle regulation. K23 plays a significant role in DNA repair mechanisms and mitotic progression by modifying proteins such as p53 and RanGAP1. These modifications are vital for the proper execution of cellular tasks and communication with related proteins that share these pathways including SUMO-1 and SUMO-2 which often compensate for each other's functions.

Dysfunction in K23 modification contributes to conditions like cancer and neurodegenerative diseases. In cancers abnormal SUMOylation mediated by K23 impacts cellular proliferation and survival pathways often involving dysregulation of proteins like p53. Neurodegenerative diseases also show altered levels of SUMOylated proteins implicating K23 in modifications that affect neuronal function and integrity. Researchers focus on these diseases to explore therapeutic potential targeting the SUMOylation pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Keratin, type I cytoskeletal 23
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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