Rabbit Polyclonal K27 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT27 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KRT27 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3Y8
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs).
Alternative names
KRT25C, KRT27, Cytokeratin-27, Keratin-25C, Keratin-27, Type I inner root sheath-specific keratin-K25irs3, CK-27, K25C, K27
Recommended products
Rabbit Polyclonal K27 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT27 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KRT27 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3Y8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The K27 target also known as lysine 27 specifically refers to the residue on histone H3 where methylation occurs playing an important role in chromatin structure. Histone H3 is approximately 15 kDa. K27 methylation takes place in the cell nucleus and its levels are regulated by enzymes such as EZH2 which is a part of the polycomb group proteins. This modification affects gene expression by altering chromatin accessibility silencing or activating different genes based on methylation state.
- Biological function summary
K27 methylation is involved in transcriptional regulation and chromatin dynamics. It is an important component of the polycomb repressive complex 2 (PRC2) which provides transcriptional repression of genes through methylation of histone H3 at lysine 27 (H3K27me3). This form of regulation is essential in maintaining the repressed state of developmentally regulated genes and in stem cell pluripotency. The dynamic balance between methylation and demethylation allows cells to respond to developmental cues and environmental changes.
- Pathways
K27 methylation is essential in the polycomb pathway involved in regulating gene expression patterns during development. This pathway provides histone modifications that result in epigenetic memory. The K27 residue interacts with proteins like Suz12 and EED within the PRC2 complex facilitating this gene repression. Additionally the K27 methylation interplays with the Trithorax pathway which has opposing functions and promotes gene activation showing the duality of gene regulation.
- Associated diseases and disorders
Aberrant regulation of K27 methylation can contribute to various forms of cancer such as breast and prostate cancer. Increased EZH2 activity which catalyzes H3K27me3 is often associated with these cancers due to inappropriate gene silencing. The dysregulation of K27 methylation might also link to PRC2 components like Suz12 making it a target for potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K27 antibody (ab220725)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human hair tissue labeling K27 with ab220725 at 1/2500 dilution.
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