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AB220725

Anti-K27 antibody

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(1 Publication)

Rabbit Polyclonal K27 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT27 aa 1-50.

View Alternative Names

KRT25C, KRT27, Cytokeratin-27, Keratin-25C, Keratin-27, Type I inner root sheath-specific keratin-K25irs3, CK-27, K25C, K27

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K27 antibody (AB220725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K27 antibody (AB220725)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human hair tissue labeling K27 with ab220725 at 1/2500 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT27 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The K27 target also known as lysine 27 specifically refers to the residue on histone H3 where methylation occurs playing an important role in chromatin structure. Histone H3 is approximately 15 kDa. K27 methylation takes place in the cell nucleus and its levels are regulated by enzymes such as EZH2 which is a part of the polycomb group proteins. This modification affects gene expression by altering chromatin accessibility silencing or activating different genes based on methylation state.
Biological function summary

K27 methylation is involved in transcriptional regulation and chromatin dynamics. It is an important component of the polycomb repressive complex 2 (PRC2) which provides transcriptional repression of genes through methylation of histone H3 at lysine 27 (H3K27me3). This form of regulation is essential in maintaining the repressed state of developmentally regulated genes and in stem cell pluripotency. The dynamic balance between methylation and demethylation allows cells to respond to developmental cues and environmental changes.

Pathways

K27 methylation is essential in the polycomb pathway involved in regulating gene expression patterns during development. This pathway provides histone modifications that result in epigenetic memory. The K27 residue interacts with proteins like Suz12 and EED within the PRC2 complex facilitating this gene repression. Additionally the K27 methylation interplays with the Trithorax pathway which has opposing functions and promotes gene activation showing the duality of gene regulation.

Aberrant regulation of K27 methylation can contribute to various forms of cancer such as breast and prostate cancer. Increased EZH2 activity which catalyzes H3K27me3 is often associated with these cancers due to inappropriate gene silencing. The dysregulation of K27 methylation might also link to PRC2 components like Suz12 making it a target for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs).
See full target information KRT27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 21:530-547 PubMed39324484

2024

SMURF1 mediates damaged lysosomal homeostasis by ubiquitinating PPP3CB to promote the activation of TFEB.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xia,Xuan Liu,Lu Zhong,Jun Qu,Lei Dong
View all publications
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Product promise

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