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AB111730

Anti-K31 antibody

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(1 Publication)

Rabbit Polyclonal K31 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT31 aa 150-400.

View Alternative Names

HHA1, HKA1, KRTHA1, KRT31, Keratin-31, K31

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-K31 antibody (AB111730)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-K31 antibody (AB111730)

Immunofluorescence analysis of K31 in methanol-fixed HeLa cells, using ab111730 at a 1/200 dilution. The lower image shows the cells counter stained with Hoechst 33342.

Western blot - Anti-K31 antibody (AB111730)
  • WB

Unknown

Western blot - Anti-K31 antibody (AB111730)

10% SDS PAGE

All lanes:

Western blot - Anti-K31 antibody (ab111730) at 1/5000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KRT31 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q15323

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Alternative Products

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K31 antibody [EPR12855(B)] (AB177955)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K31 also known as keratin 31 or KRT31 is a type I hair keratin protein. It is an essential component of the inner root sheath of hair follicles. K31 has a molecular mass of approximately 44 kDa and is selectively expressed in hair follicle cells especially in the medulla and cortex of human hair. As part of the keratin family K31 plays a role in the cytoskeleton providing structure and support to epithelial cells.
Biological function summary

Keratin 31 participates in the formation of the hair shaft and influences hair texture and integrity. It interacts with other keratin proteins forming heterodimer complexes with type II keratins such as K81 and K82 to provide mechanical stability to hair fibers. This interaction is integral for maintaining the structural integrity of hair throughout the growth cycle. The expression of K31 and its partner proteins indicates its critical role in hair fiber development and cohesion.

Pathways

Keratin 31 functions within the trichocyte keratinization pathway which is vital for hair growth and differentiation. It collaborates with other hair keratins contributing significantly to the assembly of intermediate filaments. The Wnt signaling pathway indirectly influences K31 expression by regulating keratinocyte differentiation linking K31 to other proteins such as beta-catenin. These interactions highlight the importance of K31 in hair biology.

Keratin 31 mutations or dysregulation have been connected to monilethrix a genetic hair disorder characterized by beaded hair and fragility. This condition involves abnormal keratinocyte biology highlighting K31’s association with hair structural stability issues. Furthermore altered expression of K31 is noted in certain cases of alopecia areata a condition that causes hair loss and involves an autoimmune reaction targeting hair follicles. Understanding K31’s role aids in developing treatments for these hair-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT31
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 15:488-500 PubMed33817237

2020

Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Xiaoqiang Zhou,Jing Yin,Yang Zhou
View all publications
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Product promise

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