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AB250331

Anti-K31 antibody [EPR12849] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal K31 antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

HHA1, HKA1, KRTHA1, KRT31, Keratin-31, K31

2 Images
Western blot - Anti-K31 antibody [EPR12849] - BSA and Azide free (AB250331)
  • WB

Supplier Data

Western blot - Anti-K31 antibody [EPR12849] - BSA and Azide free (AB250331)

This data was developed using ab181081, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (<a href='/en-us/products/primary-antibodies/k31-antibody-epr12849-n-terminal-ab181081'>ab181081</a>) at 1/2000 dilution

All lanes:

Rat skin lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false

Western blot - Anti-K31 antibody [EPR12849] - BSA and Azide free (AB250331)
  • WB

Supplier Data

Western blot - Anti-K31 antibody [EPR12849] - BSA and Azide free (AB250331)

This data was developed using ab181081, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (<a href='/en-us/products/primary-antibodies/k31-antibody-epr12849-n-terminal-ab181081'>ab181081</a>) at 1/10000 dilution

All lanes:

Human skin lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12849

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used to generate this antibody has 100% homology with KRT33A and KRT33B, UniProt ID's Q76009 and Q14525.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250331 is the carrier-free version of ab181081.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K31 also known as keratin 31 or KRT31 is a type I hair keratin protein. It is an essential component of the inner root sheath of hair follicles. K31 has a molecular mass of approximately 44 kDa and is selectively expressed in hair follicle cells especially in the medulla and cortex of human hair. As part of the keratin family K31 plays a role in the cytoskeleton providing structure and support to epithelial cells.
Biological function summary

Keratin 31 participates in the formation of the hair shaft and influences hair texture and integrity. It interacts with other keratin proteins forming heterodimer complexes with type II keratins such as K81 and K82 to provide mechanical stability to hair fibers. This interaction is integral for maintaining the structural integrity of hair throughout the growth cycle. The expression of K31 and its partner proteins indicates its critical role in hair fiber development and cohesion.

Pathways

Keratin 31 functions within the trichocyte keratinization pathway which is vital for hair growth and differentiation. It collaborates with other hair keratins contributing significantly to the assembly of intermediate filaments. The Wnt signaling pathway indirectly influences K31 expression by regulating keratinocyte differentiation linking K31 to other proteins such as beta-catenin. These interactions highlight the importance of K31 in hair biology.

Keratin 31 mutations or dysregulation have been connected to monilethrix a genetic hair disorder characterized by beaded hair and fragility. This condition involves abnormal keratinocyte biology highlighting K31’s association with hair structural stability issues. Furthermore altered expression of K31 is noted in certain cases of alopecia areata a condition that causes hair loss and involves an autoimmune reaction targeting hair follicles. Understanding K31’s role aids in developing treatments for these hair-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT31
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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