AB181081

Anti-K31 antibody [EPR12849] - N-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal K31 antibody. N-terminal. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

HHA1, HKA1, KRTHA1, KRT31, Keratin-31, K31

2 Images

Supplier Data

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (AB181081)

All lanes:

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (ab181081) at 1/10000 dilution

All lanes:

Human skin lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (AB181081)

All lanes:

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (ab181081) at 1/2000 dilution

All lanes:

Rat skin lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false

Carrier free

Anti-K31 antibody [EPR12849] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12849

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used to generate ab181081 has 100% homology with KRT33A and KRT33B, UniProt ID's Q76009 and Q14525.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K31 also known as keratin 31 or KRT31 is a type I hair keratin protein. It is an essential component of the inner root sheath of hair follicles. K31 has a molecular mass of approximately 44 kDa and is selectively expressed in hair follicle cells especially in the medulla and cortex of human hair. As part of the keratin family K31 plays a role in the cytoskeleton providing structure and support to epithelial cells.

Biological function summary

Keratin 31 participates in the formation of the hair shaft and influences hair texture and integrity. It interacts with other keratin proteins forming heterodimer complexes with type II keratins such as K81 and K82 to provide mechanical stability to hair fibers. This interaction is integral for maintaining the structural integrity of hair throughout the growth cycle. The expression of K31 and its partner proteins indicates its critical role in hair fiber development and cohesion.

Pathways

Keratin 31 functions within the trichocyte keratinization pathway which is vital for hair growth and differentiation. It collaborates with other hair keratins contributing significantly to the assembly of intermediate filaments. The Wnt signaling pathway indirectly influences K31 expression by regulating keratinocyte differentiation linking K31 to other proteins such as beta-catenin. These interactions highlight the importance of K31 in hair biology.

Keratin 31 mutations or dysregulation have been connected to monilethrix a genetic hair disorder characterized by beaded hair and fragility. This condition involves abnormal keratinocyte biology highlighting K31’s association with hair structural stability issues. Furthermore altered expression of K31 is noted in certain cases of alopecia areata a condition that causes hair loss and involves an autoimmune reaction targeting hair follicles. Understanding K31’s role aids in developing treatments for these hair-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT31

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:62-73 PubMed35013556

2022

The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Dragomir B Krastev,Shudong Li,Yilun Sun,Andrew J Wicks,Gwendoline Hoslett,Daniel Weekes,Luned M Badder,Eleanor G Knight,Rebecca Marlow,Mercedes Calvo Pardo,Lu Yu,Tanaji T Talele,Jiri Bartek,Jyoti S Choudhary,Yves Pommier,Stephen J Pettitt,Andrew N J Tutt,Kristijan Ramadan,Christopher J Lord

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-K31 antibody [EPR12849] - N-terminal

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (AB181081)

Western blot - Anti-K31 antibody [EPR12849] - N-terminal (AB181081)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise