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AB222074

Anti-K34 antibody - C-terminal

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Rabbit Polyclonal K34 antibody. C-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT34 aa 400 to C-terminus.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K34 antibody - C-terminal (AB222074)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K34 antibody - C-terminal (AB222074)

Paraffin-embedded human hair stained for K34 using ab222074 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-K34 antibody - C-terminal (AB222074)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-K34 antibody - C-terminal (AB222074)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for K34 (green) using ab222074 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT34 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O76011

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K34 also known as Histone H3 lysine 34 is a post-translational modification found on histone proteins. It plays a role in DNA packaging and gene expression regulation. K34 has a molecular weight of approximately 17 kDa when part of the histone complex. Researchers observe its expression in various tissues especially in actively dividing cells such as those in the bone marrow and lymphoid tissues. Its presence indicates active transcription and chromatin remodeling.
Biological function summary

This histone modification influences chromatin structure and function impacting gene expression. K34 is a part of the histone code which directs DNA-related processes like replication and repair. As part of a histone complex it interacts with other histone modifications to regulate transcription. K34 acts as a signal for recruiting chromatin remodeling complexes that facilitate the accessibility of DNA to transcription machinery.

Pathways

K34 participates in chromatin remodeling and cellular differentiation pathways. It plays a role in the epigenetic regulation of gene expression. This occurs through its interplay with other proteins like HP1 which recognizes histone modifications and modulates chromatin structure accordingly. K34 influences how transcription factors access DNA by altering chromatin conformation within regulatory pathways of gene expression.

Aberrant K34 modification associates with certain types of cancers and developmental disorders. In cancers like leukemia K34 dysregulation can disrupt normal gene expression contributing to uncontrolled cell growth. In developmental disorders alterations in K34 may affect normal cellular differentiation and tissue development. Protein interactions with K34 such as HP1 and chromatin assembly factors can influence how these diseases develop or progress due to their role in maintaining proper chromatin structure and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT34

Alternative Names

HHA4, HKA4, KRTHA4, KRT34, Keratin-34, K34
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Product promise

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