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AB222325

Anti-K80 antibody

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(3 Publications)

Rabbit Polyclonal K80 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT80 aa 350 to C-terminus.

View Alternative Names

KB20, KRT80, Cytokeratin-80, Keratin-80, Type-II keratin Kb20, CK-80, K80

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-K80 antibody (AB222325)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-K80 antibody (AB222325)

Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling K80 (green) in the intermediate filaments with ab222325 at 2 μg/ml.

Western blot - Anti-K80 antibody (AB222325)
  • WB

Supplier Data

Western blot - Anti-K80 antibody (AB222325)

Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-K80 antibody (ab222325) at 0.4 µg/mL

Lane 1:

Control siRNA transfected - MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

siRNA probe #1 transfected - MCF7 whole cell lysate

Lane 3:

siRNA probe #2 transfected - MCF7 whole cell lysate

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KRT80 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6KB66

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

K80 is a protein also known as Keratin 80. It plays a structure role in cells providing mechanical support particularly in epithelial cells. The protein has a mass of approximately 57 kDa and is expressed significantly in the skin hair follicles and nails. In these tissues K80 contributes to the keratin filament network that offers integrity and resilience to cells undergoing mechanical stress.
Biological function summary

K80 contributes to the formation of intermediate filaments which are part of the cellular cytoskeleton. These filaments provide structural stability and are essential for the differentiation of epithelial cells. K80 often functions as part of a larger keratin complex interacting with other keratins to maintain the strength and functionality of the structural network. It participates in the keratinization process important for forming the protective barrier of the skin.

Pathways

Research shows that K80 participates in the epidermal differentiation pathway. This pathway is responsible for the development and maintenance of the skin barrier. K80 interacts with other keratin proteins such as Keratin 5 and Keratin 14 to form heterodimers and filament complexes. These interactions ensure proper skin formation and response to mechanical pressures.

Abnormalities in K80 expression or function can relate to skin diseases like keratinopathic ichthyosis and hair disorders such as alopecia. Mutations in K80 disrupt the formation of keratin filaments leading to impaired skin integrity and hair structure. The protein's malfunctions might indirectly contribute to disorders through its interaction with other keratin proteins like Keratin 6 which also plays a role in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information KRT80
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40647481

2025

miR-195-5p Suppresses KRT80 Expression Inducing Cell Cycle Arrest in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Piccinno,Viviana Scalavino,Nicoletta Labarile,Giusy Bianco,Raffaele Armentano,Gianluigi Giannelli,Grazia Serino

Evidence-based complementary and alternative medicine : eCAM 2022:2630351 PubMed36248424

2022

Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-/SMAD Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Tong,Xueyuan Chen,Zhemin Feng,Changqing Xu,Yaqian Li

Scientific reports 6:24248 PubMed27053419

2016

Deficiency of the TLR4 analogue RP105 aggravates vein graft disease by inducing a pro-inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Anouk Wezel,Margreet R de Vries,Johanna M Maassen,Peter Kip,Erna A Peters,Jacco C Karper,Johan Kuiper,Ilze Bot,Paul H A Quax
View all publications
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Product promise

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