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AB251434

Anti-KA1 antibody [EPR18322] - BSA and Azide free

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Rabbit Recombinant Monoclonal KA1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

GRIK, GRIK4, GluK4, Excitatory amino acid receptor 1, Glutamate receptor KA-1, EAA1, KA1

3 Images
Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)
  • WB

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/en-us/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

All lanes:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)
  • WB

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/en-us/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)
  • WB

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/en-us/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

All lanes:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18322

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251434 is the carrier-free version of ab205537.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KA1 domain also known as the Kinase Associated-1 domain is an important structural component in certain proteins. The KA1 domain exhibits a molecular mass of approximately 8-10 kDa depending on its specific protein context. It plays an important role in targeting and binding within the cell. The KA1 domain is expressed in various cell types particularly those in the brain and muscle tissue where it contributes to cellular signaling and function.
Biological function summary

The KA1 domain of proteins helps regulate several important cellular processes. It is often part of larger protein complexes where it assists in localization and anchoring to specific structures in the cell. The domain influences various cellular activities including the assembly of signaling molecules and interaction with phospholipids. By contributing to the regulation of protein kinases and phosphatases the KA1 domain maintains critical cellular functions across different cell types.

Pathways

The KA1 domain integrates into the regulation of mitogen-activated protein kinase (MAPK) pathways and protein kinase A (PKA) signaling. These pathways play essential roles in cell growth apoptosis and other signaling processes. The KA1 domain often collaborates with other proteins such as PKC (Protein Kinase C) to maintain proper signaling cascade function and ensure accurate cellular responses.

The KA1 domain's function can link to neurodegenerative diseases and cancer. Alterations in proteins containing the KA1 domain have been associated with unregulated cell growth or improper signaling cascades often seen in cancerous tissues. The KA1 domain also interacts with proteins like tau in neurons potentially impacting neurodegenerative conditions by affecting cell signaling and structure. These interactions highlight the KA1 domain's importance in contributing to disease pathways and inform therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel. Cannot form functional channels on its own (PubMed : 8263508). Shows channel activity only in heteromeric assembly with GRIK1, GRIK2 and GRIK3 subunits (By similarity).
See full target information GRIK4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com