Anti-KACL antibody [EPR27403-26]

• WB

Supplier Data

Western blot - Anti-KACL antibody [EPR27403-26] (AB309102)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. C-type lectin domain family 2 member A is a glycoprotein of approximately 32 kDa and detected as a 20 kDa band after treated with Protein Deglycosylation MIX II. The expression molecular weight observed is consistent with what has been described in the literature (PMID : 25510854).

All lanes:

Western blot - Anti-KACL antibody [EPR27403-26] (ab309102) at 1/1000 dilution

Lane 1:

Untreated U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

U-937 whole cell lysate treated with Protein Deglycosylation MIXII at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 32 kDa,20 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KACL antibody [EPR27403-26] (AB309102)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : THP-1 and HeLa (PMID : 18046548). The identity of the higher MW band at approximately 60 kDa (in lane 3) is unknown. In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution.

All lanes:

Western blot - Anti-KACL antibody [EPR27403-26] (ab309102) at 1/1000 dilution

Lane 1:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 32 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-KACL antibody [EPR27403-26] (AB309102)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KACL antibody [EPR27403-26] (ab309102) at 1/1000 dilution

All lanes:

Human skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Observed band size: 32 kDa

true

Exposure time: 180s