AB124777

Anti-Kaiso antibody [EPR4668]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal KAISO antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

KAISO, ZNF348, ZBTB33, Transcriptional regulator Kaiso, Zinc finger and BTB domain-containing protein 33

1 Images

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Western blot - Anti-Kaiso antibody [EPR4668] (AB124777)

All lanes:

Western blot - Anti-Kaiso antibody [EPR4668] (ab124777) at 1/1000 dilution

Lane 1:

Human heart lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Lane 3:

C6 lysate at 10 µg

Lane 4:

PC12 lysate at 10 µg

Lane 5:

NIH 3T3 lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 95 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4668

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kaiso also known as ZBTB33 is a transcriptional regulator with a mass of approximately 75 kilodaltons. It is a member of the POZ-zinc finger family of proteins. Researchers identified Kaiso as a dual-specificity transcription factor meaning it can bind to both DNA methylated and non-methylated sequences. It is widely expressed in various tissues including the brain liver heart and testis. This broad expression pattern indicates its potential involvement in multiple cellular processes.

Biological function summary

Kaiso functions as a transcriptional repressor influencing gene expression by binding to specific DNA sequences. It operates as part of a protein complex that includes other corepressors like N-CoR and SMRT. These interactions modulate chromatin structure which affects gene accessibility. Kaiso's repression of target genes is essential for maintaining cellular homeostasis. Research shows that Kaiso also interacts with p120-catenin a protein involved in cell adhesion linking its function to cytoskeletal dynamics and cell motility.

Pathways

Several studies show that Kaiso is part of key signaling cascades such as the Wnt/β-catenin pathway. It interacts with other pathway regulators including TCF/LEF transcription factors and is influenced by β-catenin signaling which plays an important role in embryonic development and cellular proliferation. Another significant pathway involving Kaiso is the Notch signaling pathway where Kaiso's function contributes to cell fate determination and differentiation. Through these pathways its ability to regulate gene expression impacts many biological processes.

Kaiso has been linked to cancer progression and metastasis. Its downregulation or loss of function can lead to tumor growth primarily due to the deregulation of genes involved in cell proliferation and apoptosis. Studies highlight its association with colorectal cancer where it influences the expression of tumor suppressor genes. Additionally Kaiso's interaction with β-catenin connects it to other diseases like colorectal cancer where dysregulation of the Wnt pathway plays a critical role. These connections emphasize the potential impact of Kaiso on disease pathogenesis and its value as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed : 23251453).

See full target information ZBTB33

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular physiology and biochemistry : internation 48:947-958 PubMed30036882

2018

Kaiso (ZBTB33) Downregulation by Mirna-181a Inhibits Cell Proliferation, Invasion, and the Epithelial-Mesenchymal Transition in Glioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Wang,Jichao Ma,Xiaoxiong Wang,Fei Peng,Xin Chen,Bingjie Zheng,Chunlei Wang,Zhibo Dai,Jing Ai,Shiguang Zhao

Oncology research 27:325-334 PubMed29716673

2018

Overexpression of miR-1283 Inhibits Cell Proliferation and Invasion of Glioma Cells by Targeting ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Chen,Yi Zhang,Hai Su,Hui Shi,Qijiang Xiong,Zulu Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kaiso antibody [EPR4668]

Western blot - Anti-Kaiso antibody [EPR4668] (AB124777)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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