Rabbit Polyclonal Kallikrein 14 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLK14 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KLK14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0G3
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.
Alternative names
KLKL6, KLK14, Kallikrein-14, hK14, Kallikrein-like protein 6, KLK-L6
Recommended products
Rabbit Polyclonal Kallikrein 14 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLK14 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KLK14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0G3
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kallikrein 14 (KLK14) a serine protease is involved in various proteolytic activities. It has a molecular mass of approximately 25 kDa. KLK14 is part of the kallikrein-related peptidase family and is expressed in diverse tissues including skin prostate and brain. This protein is also known by the alternate name hK14. The expression pattern of KLK14 suggests its involvement in local tissue-specific functions.
- Biological function summary
KLK14 influences processes like skin desquamation and seminal plasma liquefaction. It plays a significant role in the regulation of extracellular matrix components and participates in activating protein precursors. Though it functions as an individual protease KLK14 interacts with other kallikreins in a possible cascade-like manner indicating complex interplay between these proteins.
- Pathways
KLK14 is key in processes involving the kallikrein-kinin system and the activation of certain growth factors. It can cleave and activate other proteins such as renin-angiotensin system components influencing blood pressure regulation. KLK14 operates within this intricate network alongside proteins like KLK1 and other kallikreins that have complementary roles in physiological pathways.
- Associated diseases and disorders
KLK14 has been linked to prostate cancer and skin-related conditions such as atopic dermatitis. Overexpression of KLK14 correlates with prostate cancer progression suggesting its involvement in tumorigenesis. It is also associated with other kallikreins like KLK3 (PSA) in cancer influencing disease development and prognosis. In skin disorders KLK14 may affect processes like inflammation and barrier function revealing its therapeutic target potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody (ab232966)
Formalin-fixed, paraffin-embedded human liver tissue stained for Kallikrein 14 using ab232966 at 10 μg/mL in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody (ab232966)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Kallikrein 14 using ab232966 at 10 μg/mL in immunohistochemical analysis. DAB staining.
Western blot - Anti-Kallikrein 14 antibody (ab232966)
All lanes: Western blot - Anti-Kallikrein 14 antibody (ab232966) at 1 µg/mL
All lanes: Human lung tissue lysate
SecondaryAll lanes: HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL
Predicted band size: 29 kDa
Western blot - Anti-Kallikrein 14 antibody (ab232966)
All lanes: Western blot - Anti-Kallikrein 14 antibody (ab232966) at 1 µg/mL
All lanes: Pig kidney tissue lysate
SecondaryAll lanes: HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL
Predicted band size: 29 kDa
Western blot - Anti-Kallikrein 14 antibody (ab232966)
All lanes: Western blot - Anti-Kallikrein 14 antibody (ab232966) at 2 µg/mL
All lanes: Recombinant human Kallikrein 14 protein
Predicted band size: 29 kDa
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