AB128957

Anti-Kallikrein 14 antibody [EPR7209]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Kallikrein 14 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KLKL6, KLK14, Kallikrein-14, hK14, Kallikrein-like protein 6, KLK-L6

2 Images

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Western blot - Anti-Kallikrein 14 antibody [EPR7209] (AB128957)

All lanes:

Western blot - Anti-Kallikrein 14 antibody [EPR7209] (ab128957) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

Fetal kidney lysate at 10 µg

Lane 4:

HACAT cell lysate at 10 µg

Lane 5:

Prostate cancer lysate at 10 µg

Predicted band size: 29 kDa

Observed band size: 29 kDa,31 kDa

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OI-RD Scanning - Anti-Kallikrein 14 antibody [EPR7209] (AB128957)

OI-RD Scanning

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OI-RD Scanning - Anti-Kallikrein 14 antibody [EPR7209] (AB128957)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7209

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 14 (KLK14) a serine protease is involved in various proteolytic activities. It has a molecular mass of approximately 25 kDa. KLK14 is part of the kallikrein-related peptidase family and is expressed in diverse tissues including skin prostate and brain. This protein is also known by the alternate name hK14. The expression pattern of KLK14 suggests its involvement in local tissue-specific functions.

Biological function summary

KLK14 influences processes like skin desquamation and seminal plasma liquefaction. It plays a significant role in the regulation of extracellular matrix components and participates in activating protein precursors. Though it functions as an individual protease KLK14 interacts with other kallikreins in a possible cascade-like manner indicating complex interplay between these proteins.

Pathways

KLK14 is key in processes involving the kallikrein-kinin system and the activation of certain growth factors. It can cleave and activate other proteins such as renin-angiotensin system components influencing blood pressure regulation. KLK14 operates within this intricate network alongside proteins like KLK1 and other kallikreins that have complementary roles in physiological pathways.

KLK14 has been linked to prostate cancer and skin-related conditions such as atopic dermatitis. Overexpression of KLK14 correlates with prostate cancer progression suggesting its involvement in tumorigenesis. It is also associated with other kallikreins like KLK3 (PSA) in cancer influencing disease development and prognosis. In skin disorders KLK14 may affect processes like inflammation and barrier function revealing its therapeutic target potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.

See full target information KLK14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 137:377-384 PubMed27765722

2016

Staphylococcus aureus Induces Increased Serine Protease Activity in Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael R Williams,Teruaki Nakatsuji,James A Sanford,Alison F Vrbanac,Richard L Gallo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 14 antibody [EPR7209]

Western blot - Anti-Kallikrein 14 antibody [EPR7209] (AB128957)

OI-RD Scanning - Anti-Kallikrein 14 antibody [EPR7209] (AB128957)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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