Rabbit Polyclonal Kallikrein 2/KLK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20151
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Alternative names
Kallikrein-2, Glandular kallikrein-1, Tissue kallikrein-2, hGK-1, KLK2
Recommended products
Rabbit Polyclonal Kallikrein 2/KLK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20151
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Kallikrein 2 also known as KLK2 or kallikrein-related peptidase 2 is a serine protease with a molecular mass of approximately 28 kDa. KLK2 is predominantly expressed in the prostate gland and plays a role in the cleavage of specific protein substrates. It shares homology with other kallikrein family proteins and is involved in the enzymatic activity that regulates various physiological processes. This protein also carries the alternate names like ALSK24680 KLK2 protein and has a significant presence in seminal plasma.
- Biological function summary
KLK2 is involved in the regulation of seminal fluid by activating other enzymes such as prostate-specific antigen (PSA) which is itself part of the kallikrein family. KLK2 functions in the liquefaction process of seminal fluid aiding in sperm motility. It exists as part of a proteolytic cascade complex which includes other kallikreins. The expression and activity of KLK2 are highly regulated by androgens in the prostate tissue indicating its importance in male reproductive health.
- Pathways
KLK2 participates in the kallikrein-kinin system and is closely related to other kallikrein family members like KLK3 (PSA) in regulatory mechanisms critical for reproductive biology. KLK2 influences pathways such as the fibrinolysis system by activating urokinase-type plasminogen activator (uPA). Additionally KLK2 modulates pathways related to extracellular matrix degradation connecting with proteins such as matrix metalloproteinases (MMPs).
- Associated diseases and disorders
KLK2 has a strong association with prostate cancer often used as a biomarker alongside PSA for early detection and prognosis assessment. Elevated levels of KLK2 can indicate a progression of malignant disease in the prostate. Furthermore KLK2 may have connections to benign prostatic hyperplasia (BPH) a noncancerous enlargement of the prostate. Its expression levels and activity can influence disease development and it is considered in research targeting prostate-related health disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136)
12% SDS PAGE
All lanes: Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136) at 1/500 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (ab152136)
Immunohistochemical analysis of paraffin-embedded AIHC xenograft labeling Kallikrein 2/KLK2 with ab152136 at 1/100 dilution.
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