AB152136

Anti-Kallikrein 2/KLK2 antibody

Name: Anti-Kallikrein 2/KLK2 antibody (ab152136) | Abcam
Brand: Abcam Limited
SKU: AB152136

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(3 Publications)

Rabbit Polyclonal Kallikrein 2/KLK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus.

View Alternative Names

Kallikrein-2, Glandular kallikrein-1, Tissue kallikrein-2, hGK-1, KLK2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Immunohistochemical analysis of paraffin-embedded human prostate and human salivary gland tissues labeling Kallikrein 2/KLK2 with ab152136 at 1/1000 dilution.

Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

15% SDS-PAGE

The signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136) at 1/500 dilution

All lanes:

22Rv1 (Human prostate carcinoma epithelial cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

15% SDS-PAGE

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136) at 1/500 dilution

All lanes:

Human prostate tissue extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KLK2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Kallikrein 2 also known as KLK2 or kallikrein-related peptidase 2 is a serine protease with a molecular mass of approximately 28 kDa. KLK2 is predominantly expressed in the prostate gland and plays a role in the cleavage of specific protein substrates. It shares homology with other kallikrein family proteins and is involved in the enzymatic activity that regulates various physiological processes. This protein also carries the alternate names like ALSK24680 KLK2 protein and has a significant presence in seminal plasma.

Biological function summary

KLK2 is involved in the regulation of seminal fluid by activating other enzymes such as prostate-specific antigen (PSA) which is itself part of the kallikrein family. KLK2 functions in the liquefaction process of seminal fluid aiding in sperm motility. It exists as part of a proteolytic cascade complex which includes other kallikreins. The expression and activity of KLK2 are highly regulated by androgens in the prostate tissue indicating its importance in male reproductive health.

Pathways

KLK2 participates in the kallikrein-kinin system and is closely related to other kallikrein family members like KLK3 (PSA) in regulatory mechanisms critical for reproductive biology. KLK2 influences pathways such as the fibrinolysis system by activating urokinase-type plasminogen activator (uPA). Additionally KLK2 modulates pathways related to extracellular matrix degradation connecting with proteins such as matrix metalloproteinases (MMPs).

KLK2 has a strong association with prostate cancer often used as a biomarker alongside PSA for early detection and prognosis assessment. Elevated levels of KLK2 can indicate a progression of malignant disease in the prostate. Furthermore KLK2 may have connections to benign prostatic hyperplasia (BPH) a noncancerous enlargement of the prostate. Its expression levels and activity can influence disease development and it is considered in research targeting prostate-related health disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

See full target information KLK2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400398 PubMed38958553

2024

UBX-390: A Novel Androgen Receptor Degrader for Therapeutic Intervention in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soohyun Lee,Hwa-Ryeon Kim,Yaejin Woo,Jiyoung Kim,Han Wool Kim,Ji Youn Park,Beomseon Suh,Yuri Choi,Jungmin Ahn,Je Ho Ryu,Jae-Seok Roe,Jaewhan Song,Song Hee Lee

Molecular oncology 18:1966-1979 PubMed38605607

2024

Manzamine A reduces androgen receptor transcription and synthesis by blocking E2F8-DNA interactions and effectively inhibits prostate tumor growth in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dev Karan,Seema Dubey,Sumedha Gunewardena,Kenneth A Iczkowski,Manohar Singh,Pengyuan Liu,Angelo Poletti,Yeun-Mun Choo,Hui-Zi Chen,Mark T Hamann

The Journal of clinical investigation 133: PubMed37022795

2023

Long noncoding RNA HIKER regulates erythropoiesis in Monge's disease via CSNK2B.

Applications

Unspecified application

Species

Unspecified reactive species

Priti Azad,Dan Zhou,Hung-Chi Tu,Francisco C Villafuerte,David Traver,Tariq M Rana,Gabriel G Haddad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 2/KLK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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