AB232980

Anti-Kallikrein 2/KLK2 antibody

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(1 Publication)

Rabbit Polyclonal Kallikrein 2/KLK2 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLK2.

View Alternative Names

Kallikrein-2, Glandular kallikrein-1, Tissue kallikrein-2, hGK-1, KLK2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human glioma tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB232980)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB232980)

WB

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Western blot - Anti-Kallikrein 2/KLK2 antibody (AB232980)

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab232980) at 2 µg/mL

All lanes:

Rat prostate gland tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB232980)

WB

Supplier Data

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB232980)

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab232980) at 2 µg/mL

All lanes:

Recombinant human Kallikrein 2/KLK2 protein

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

Immunogen

Recombinant Full Length Protein corresponding to Human KLK2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Kallikrein 2 also known as KLK2 or kallikrein-related peptidase 2 is a serine protease with a molecular mass of approximately 28 kDa. KLK2 is predominantly expressed in the prostate gland and plays a role in the cleavage of specific protein substrates. It shares homology with other kallikrein family proteins and is involved in the enzymatic activity that regulates various physiological processes. This protein also carries the alternate names like ALSK24680 KLK2 protein and has a significant presence in seminal plasma.

Biological function summary

KLK2 is involved in the regulation of seminal fluid by activating other enzymes such as prostate-specific antigen (PSA) which is itself part of the kallikrein family. KLK2 functions in the liquefaction process of seminal fluid aiding in sperm motility. It exists as part of a proteolytic cascade complex which includes other kallikreins. The expression and activity of KLK2 are highly regulated by androgens in the prostate tissue indicating its importance in male reproductive health.

Pathways

KLK2 participates in the kallikrein-kinin system and is closely related to other kallikrein family members like KLK3 (PSA) in regulatory mechanisms critical for reproductive biology. KLK2 influences pathways such as the fibrinolysis system by activating urokinase-type plasminogen activator (uPA). Additionally KLK2 modulates pathways related to extracellular matrix degradation connecting with proteins such as matrix metalloproteinases (MMPs).

KLK2 has a strong association with prostate cancer often used as a biomarker alongside PSA for early detection and prognosis assessment. Elevated levels of KLK2 can indicate a progression of malignant disease in the prostate. Furthermore KLK2 may have connections to benign prostatic hyperplasia (BPH) a noncancerous enlargement of the prostate. Its expression levels and activity can influence disease development and it is considered in research targeting prostate-related health disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

See full target information KLK2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102380 PubMed39640012

2024

A lipid nanoparticle-based oligodendrocyte-specific mRNA therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Sawamura,Kiyoshi Tachikawa,Rie Hikawa,Hisako Akiyama,Seiji Kaji,Ken Yasuda,Angel I Leu,Hyojung Hong,Rajesh Mukthavaram,Pad Chivukula,Hodaka Yamakado,Yoshio Hirabayashi,Ryosuke Takahashi,Shu-Ichi Matsuzawa

View all publications

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Product promise

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