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AB28565

Anti-Kallikrein 5 antibody

3

(1 Review)

|

(5 Publications)

Rabbit Polyclonal Kallikrein 5 antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KLK5.

View Alternative Names

SCTE, UNQ570/PRO1132, KLK5, Kallikrein-5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme, KLK-L2

1 Images
Western blot - Anti-Kallikrein 5 antibody (AB28565)
  • WB

Unknown

Western blot - Anti-Kallikrein 5 antibody (AB28565)

All lanes:

Western blot - Anti-Kallikrein 5 antibody (ab28565) at 1/1000 dilution

Lane 1:

Kallikrein 5 at 0.05 µg

Lane 2:

Kallikrein 5 at 0.01 µg

Lane 3:

Kallikrein 5 at 0.001 µg

Predicted band size: 32 kDa

Observed band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Synthetic Peptide within Human KLK5. The exact immunogen used to generate this antibody is proprietary information.

Q9Y337

Specificity

This antibody reacts with Kallikrein 5, but does not react with the other Kallikreins (1-7 and 9-15).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1000 (when using colorimetric substrates such as BCIP/NBT)- 1/5000 (chemiluminescent substrates). Dilution optimised using Chromogenic detection.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 5 also known as KLK5 or stratum corneum tryptic enzyme is a serine protease enzyme with a molecular weight of approximately 30 kDa. It is encoded by the KLK5 gene and is part of the kallikrein family. KLK5 is mainly expressed in the skin but also found in other tissues such as the esophagus and breast. The enzyme plays a mechanical role in skin barrier function by processing and activating other kallikreins catalyzing the degradation of corneodesmosomes which are essential for skin desquamation.
Biological function summary

KLK5 assists in maintaining the skin's outermost layer by regulating the desquamation process. It does not operate in isolation but is an intrinsic part of the kallikrein proteolytic cascade which involves several other kallikrein-related peptidases including KLK7 and KLK14. This cascade ensures proper turnover and shedding of damaged or mature cells from the skin surface playing an important role in skin homeostasis and renewal. It is significant in forming a protective barrier against environmental insults.

Pathways

KLK5 integrates into the skin barrier and inflammation regulation pathways. Its activity influences the activation of other proteases and signaling molecules involved in skin inflammation and repair processes. Related proteins like protease-activated receptors (PARs) interact with KLK5 mediating downstream signaling that impacts inflammation and tissue remodeling. The cascades involving KLK5 serve as checkpoints in cutaneous homeostasis and immune responses.

KLK5 has an important association with skin conditions like atopic dermatitis and Netherton syndrome. In atopic dermatitis abnormal KLK5 expression leads to compromised skin barrier integrity exacerbating the condition. It also relates to LEKTI (lympho-epithelial Kazal-type related inhibitor) dysfunction in Netherton syndrome where uncontrolled KLK5 activity contributes to severe barrier defects. The interactions between KLK5 and these disorders highlight its role in pathophysiological processes affecting skin health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in desquamation.
See full target information KLK5
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical laboratory analysis 35:e23958 PubMed34510543

2021

Clinical significance of kallikrein 5 as a novel prognostic biomarker in gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokaiti Abuduhadeer,Xincai Xu,Kamali Aihesan,Maimaiti Yilihamu,Yanjun Zhao,Wenbin Zhang

Translational oncology 14:100970 PubMed33260070

2020

Relative expression of KLK5 to LEKTI is associated with aggressiveness of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Márcia Gaião Alves,Márcio Hideki Kodama,Elaine Zayas Marcelino da Silva,Bruno Belmonte Martinelli Gomes,Rodrigo Alberto Alves da Silva,Gabriel Viliod Vieira,Vani Maria Alves,Carol Kobori da Fonseca,Ana Carolina Santana,Nerry Tatiana Cecílio,Mara Silvia Alexandre Costa,Maria Célia Jamur,Constance Oliver,Thiago Mattar Cunha,Thomas H Bugge,Paulo Henrique Braz-Silva,Leandro M Colli,Katiuchia Uzzun Sales

The Journal of biological chemistry 286:9127-35 PubMed21163944

2010

Kallikrein-5 promotes cleavage of desmoglein-1 and loss of cell-cell cohesion in oral squamous cell carcinoma.

Applications

ICC/IF

Species

Human

Rong Jiang,Zonggao Shi,Jeffrey J Johnson,Yueying Liu,M Sharon Stack

Molecular cancer research : MCR 8:145-58 PubMed20145038

2010

Urinary-type plasminogen activator receptor/alpha 3 beta 1 integrin signaling, altered gene expression, and oral tumor progression.

Applications

IHC-P

Species

Mouse

Supurna Ghosh,Jennifer Koblinski,Jeffrey Johnson,Yueying Liu,Aaron Ericsson,J Wade Davis,Zonggao Shi,Matthew J Ravosa,Susan Crawford,Shellaine Frazier,M Sharon Stack

Histology and histopathology 24:197-207 PubMed19085836

2008

Multiple kallikrein (KLK 5, 7, 8, and 10) expression in squamous cell carcinoma of the oral cavity.

Applications

Unspecified application

Species

Unspecified reactive species

Jason R Pettus,Jeffrey J Johnson,Zonggao Shi,J Wade Davis,Jennifer Koblinski,Supurna Ghosh,Yueying Liu,Matthew J Ravosa,Shellaine Frazier,M Sharon Stack
View all publications
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