Rabbit Polyclonal Kallikrein 6 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KLK6 aa 200 to C-terminus.
Images
Publications
- Matrix biology plus 6-7:1000192019Kallikreins: Essential epidermal messengers for regulation of the skin microenvironment during homeostasis, repair and disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPauline Nauroy,Alexander NyströmPubMed 33543017
- Journal of gastric cancer 18:356-3672018KLK6 Promotes Growth, Migration, and Invasion of Gastric Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengxing Zhu,Jihua Shi,Shanfeng Zhang,Zhen LiPubMed 30607299
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human KLK6 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92876
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
Alternative names
Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme, PRSS9, PRSS18, KLK6
Recommended products
Rabbit Polyclonal Kallikrein 6 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KLK6 aa 200 to C-terminus.
Alternative names
Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme, PRSS9, PRSS18, KLK6
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human KLK6 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q92876
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
Kallikrein 6 also known as KLK6 is a serine protease with notable enzymatic properties. This protein part of the kallikrein family weighs approximately 25 kDa and exhibits chymotrypsin-like activity. KLK6 is highly expressed in the central nervous system and various body fluids such as cerebrospinal fluid and milk. Due to this distribution KLK6 may play roles in neurological and physiological processes.
- Biological function summary
KLK6 participates in the degradation of extracellular matrix components. It functions within complex proteolytic cascades facilitating tissue remodeling and repair. Additionally KLK6 interacts with other kallikreins like kallikrein 3 showing intricate regulation within the kallikrein-related peptidase network. This interplay points toward its importance in modulating extracellular environment and cellular interactions.
- Pathways
KLK6 engages in the neurogenic and inflammatory response pathways. It ties into MAPK signaling influencing processes such as cell proliferation and apoptosis. Through these pathways KLK6 associates with proteins like MMPs which modulate extracellular matrix dynamics. Its presence in these pathways positions KLK6 as a significant regulatory protein during cellular homeostasis and stress responses.
- Associated diseases and disorders
KLK6 correlates with neurodegenerative diseases like Alzheimer's and multiple sclerosis. Altered expression and activity levels of KLK6 have implications for disease progression and pathology. In Alzheimer's KLK6 may influence amyloid-beta processing connecting with proteins like APP. In multiple sclerosis KLK6 affects demyelination processes showing potential interactions with myelin-associated proteins. Understanding these relationships can guide therapeutic strategies targeting KLK6-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Kallikrein 6 antibody - C-terminal (ab190924)
All lanes: Western blot - Anti-Kallikrein 6 antibody - C-terminal (ab190924) at 0.5 µg/mL
Lane 1: MCF-7 whole cell lysate at 40 µg
Lane 2: HeLa whole cell lysate at 40 µg
Lane 3: MM 231 whole cell lysate at 40 µg
Lane 4: MM 453 whole cell lysate at 40 µg
Lane 5: A549 whole cell lysate at 40 µg
Lane 6: SMMC whole cell lysate at 40 µg
Lane 7: COLO320 whole cell lysate at 40 µg
Lane 8: SW620 whole cell lysate at 40 µg
Lane 9: HT1080 whole cell lysate at 40 µg
Predicted band size: 27 kDa
Observed band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 6 antibody - C-terminal (ab190924)
Immunohistochemical analysis of formalin fixed paraffin-embedded Human intestinal cancer tissue labeling Kallikrein 6 using ab190924 at 1 μg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 6 antibody - C-terminal (ab190924)
Immunocytochemical analysis of PC3 cells labeling Kallikrein 6 using ab190924 at 1 μg/ml.
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