AB244367

Anti-Kallikrein 7/KLK7 antibody

Name: Anti-Kallikrein 7/KLK7 antibody (ab244367) | Abcam
Brand: Abcam Limited
SKU: AB244367
Price: 630 USD
Availability: InStock

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Rabbit Polyclonal Kallikrein 7/KLK7 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLK7 aa 100 to C-terminus.

View Alternative Names

PRSS6, SCCE, KLK7, Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 7/KLK7 antibody (AB244367)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 7/KLK7 antibody (AB244367)

PFA fixed, Triton X-100 permeabilized HaCat (Human keratinocyte cell line) cells labeling Kallikrein 7/KLK7 using ab244367 at 4 μg/ml (green) in ICC/IF.

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Western blot - Anti-Kallikrein 7/KLK7 antibody (AB244367)

All lanes:

Western blot - Anti-Kallikrein 7/KLK7 antibody (ab244367) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

Kallikrein 7/KLK7 overexpression HEK-293T cell lysate

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human KLK7 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49862

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 7 also known as KLK7 is a serine protease enzyme active in the skin. The protein's mass is approximately 27 kDa. It helps in the desquamation process by breaking down corneodesmosomes which are cell structures that facilitate cell adhesion in the outer skin layer. Kallikrein 7 is highly expressed in the epidermis and various mucosal surfaces reflecting its essential role in maintaining skin barrier functions.

Biological function summary

KLK7 contributes to skin homeostasis and renewal processes. Its enzymatic action involves cleaving proteins like desmoglein and corneodesmosin which are part of the desmosomal complex. This activity promotes the natural shedding of skin cells a process important for skin turnover and repair. By regulating this balance KLK7 supports not only skin health but also its resilience against external stressors.

Pathways

KLK7 plays a significant role in processes such as the proteolytic cascade and inflammation. It is involved in the kallikrein-kinin system alongside proteins like KLK5 that modulate inflammatory responses and barrier function in skin. KLK7 also interacts with pathways related to tissue remodeling influencing processes like inflammation through cytokine activation.

KLK7 is linked to skin conditions like atopic dermatitis and psoriasis. These disorders often feature a disrupted skin barrier where KLK7-mediated proteolytic activity increases and may exacerbate inflammation. The relationship with inflammation also connects KLK7 to proteins such as KLK5 which can further intensify skin disorders when their activities are unregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

See full target information KLK7

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 7/KLK7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 7/KLK7 antibody (AB244367)

Western blot - Anti-Kallikrein 7/KLK7 antibody (AB244367)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise