AB96710

Anti-Kallikrein 7/KLK7 antibody

Name: Anti-Kallikrein 7/KLK7 antibody (ab96710) | Abcam
Brand: Abcam Limited
SKU: AB96710

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(4 Publications)

Rabbit Polyclonal Kallikrein 7/KLK7 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLK7 aa 1 to C-terminus.

View Alternative Names

PRSS6, SCCE, KLK7, Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Immunohistochemical analysis of paraffin-embedded Cal27 using ab96710 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labelling secreted Kallikrein 7 protein with ab96710 at a 1/1000 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Kallikrein 7/KLK7 antibody (ab96710) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Kallikrein 7 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KLK7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49862

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/10000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 7 also known as KLK7 is a serine protease enzyme active in the skin. The protein's mass is approximately 27 kDa. It helps in the desquamation process by breaking down corneodesmosomes which are cell structures that facilitate cell adhesion in the outer skin layer. Kallikrein 7 is highly expressed in the epidermis and various mucosal surfaces reflecting its essential role in maintaining skin barrier functions.

Biological function summary

KLK7 contributes to skin homeostasis and renewal processes. Its enzymatic action involves cleaving proteins like desmoglein and corneodesmosin which are part of the desmosomal complex. This activity promotes the natural shedding of skin cells a process important for skin turnover and repair. By regulating this balance KLK7 supports not only skin health but also its resilience against external stressors.

Pathways

KLK7 plays a significant role in processes such as the proteolytic cascade and inflammation. It is involved in the kallikrein-kinin system alongside proteins like KLK5 that modulate inflammatory responses and barrier function in skin. KLK7 also interacts with pathways related to tissue remodeling influencing processes like inflammation through cytokine activation.

KLK7 is linked to skin conditions like atopic dermatitis and psoriasis. These disorders often feature a disrupted skin barrier where KLK7-mediated proteolytic activity increases and may exacerbate inflammation. The relationship with inflammation also connects KLK7 to proteins such as KLK5 which can further intensify skin disorders when their activities are unregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

See full target information KLK7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of the European Academy of Dermatology and Venereology : JEADV 37:605-614 PubMed36367625

2022

An open source pipeline for quantitative immunohistochemistry image analysis of inflammatory skin disease using artificial intelligence.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchun Ding,Gaurav Dhawan,Claire Jones,Thomas Ness,Esme Nichols,Natalio Krasnogor,Nick J Reynolds

Organic & biomolecular chemistry 19:6834-6841 PubMed34308939

2021

Generation of a quenched phosphonate activity-based probe for labelling the active KLK7 protease.

Applications

Unspecified application

Species

Unspecified reactive species

Evangelos Bisyris,Eleni Zingkou,Golfo G Kordopati,Minos Matsoukas,Plato A Magriotis,Georgios Pampalakis,Georgia Sotiropoulou

Molecular medicine reports 23: PubMed33537832

2021

miR‑27a‑3p regulates the inhibitory influence of endothelin 3 on the tumorigenesis of papillary thyroid cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxin Chen,Binlin Cai,Kun Liu,Qingquan Hua

Matrix biology plus 6-7:100019 PubMed33543017

2019

Kallikreins: Essential epidermal messengers for regulation of the skin microenvironment during homeostasis, repair and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Nauroy,Alexander Nyström

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 7/KLK7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Western blot - Anti-Kallikrein 7/KLK7 antibody (AB96710)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise