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AB281653

Anti-Kallikrein 7/KLK7 antibody [EPR24515-24] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Kallikrein 7/KLK7 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

PRSS6, SCCE, KLK7, Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE

1 Images
Sandwich ELISA - Anti-Kallikrein 7/KLK7 antibody [EPR24515-24] - BSA and Azide free (Detector) (AB281653)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Kallikrein 7/KLK7 antibody [EPR24515-24] - BSA and Azide free (Detector) (AB281653)

Example of Human Kallikrein 7/KLK7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24515-24

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281653 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PML. The recommended pair for sandwich ELISA is:
Capture: ab281654, Human Kallikrein 7/KLK7 Capture Antibody (unconjugated)
Detector: ab281653, Human Kallikrein 7/KLK7 Detector Antibody (unconjugated)
The reference range value is 0.156 - 10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 7 also known as KLK7 is a serine protease enzyme active in the skin. The protein's mass is approximately 27 kDa. It helps in the desquamation process by breaking down corneodesmosomes which are cell structures that facilitate cell adhesion in the outer skin layer. Kallikrein 7 is highly expressed in the epidermis and various mucosal surfaces reflecting its essential role in maintaining skin barrier functions.
Biological function summary

KLK7 contributes to skin homeostasis and renewal processes. Its enzymatic action involves cleaving proteins like desmoglein and corneodesmosin which are part of the desmosomal complex. This activity promotes the natural shedding of skin cells a process important for skin turnover and repair. By regulating this balance KLK7 supports not only skin health but also its resilience against external stressors.

Pathways

KLK7 plays a significant role in processes such as the proteolytic cascade and inflammation. It is involved in the kallikrein-kinin system alongside proteins like KLK5 that modulate inflammatory responses and barrier function in skin. KLK7 also interacts with pathways related to tissue remodeling influencing processes like inflammation through cytokine activation.

KLK7 is linked to skin conditions like atopic dermatitis and psoriasis. These disorders often feature a disrupted skin barrier where KLK7-mediated proteolytic activity increases and may exacerbate inflammation. The relationship with inflammation also connects KLK7 to proteins such as KLK5 which can further intensify skin disorders when their activities are unregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
See full target information KLK7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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