AB232839

Anti-Kallikrein 8/KLK8 antibody

Name: Anti-Kallikrein 8/KLK8 antibody (ab232839) | Abcam
Brand: Abcam Limited
SKU: AB232839

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(4 Publications)

Rabbit Polyclonal Kallikrein 8/KLK8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLK8.

View Alternative Names

NRPN, PRSS19, TADG14, UNQ283/PRO322, KLK8, Kallikrein-8, hK8, Neuropsin, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, NP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody (AB232839)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody (AB232839)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Kallikrein 8/KLK8 using ab232839 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Kallikrein 8/KLK8 antibody (AB232839)

All lanes:

Western blot - Anti-Kallikrein 8/KLK8 antibody (ab232839) at 1 µg/mL

All lanes:

Mouse ear lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-Kallikrein 8/KLK8 antibody (AB232839)

All lanes:

Western blot - Anti-Kallikrein 8/KLK8 antibody (ab232839) at 2 µg/mL

All lanes:

Recombinant human Kallikrein 8/KLK8 protein

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KLK8.

O60259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232839 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 8 also known as KLK8 is a serine protease enzyme with a molecular mass of approximately 28 kDa. Alternative names include Neurosin and hK8. It is expressed in various tissues notably in the brain testis and skin. KLK8 plays a mechanical role in cleaving protein substrates in these regions aiding in maintaining cellular structures and processes through its proteolytic activity. Its expression is important in neuronal development making it significant in central nervous system function.

Biological function summary

KLK8 involves itself in tissue remodeling and neural plasticity. It interacts with extracellular matrix proteins contributing to cell migration and adhesion. Important in synaptic plasticity KLK8 supports memory formation and learning. KLK8 operates as part of proteolytic cascades and may overlap with other kallikreins in function. Its activity includes modulating neural and skin cells suggesting its importance in cellular differentiation and growth.

Pathways

KLK8 takes part in the kallikrein-kinin system and extracellular matrix degradation pathways. It interacts with proteins such as KLK3 (prostate-specific antigen) within these pathways although KLK3 is more associated with prostate tissue. KLK8's function in these pathways ensures proper maintenance and remodeling of tissue-specific extracellular environments. These activities highlight its relevance in maintaining physiological balance and cellular responses in its primary tissues of expression.

KLK8 links with neurological conditions and certain skin disorders. Altered levels or dysfunctions of KLK8 correlate with disorders like Alzheimer's disease indicating its potential role in amyloid processing. Similar associations exist with skin disorders like psoriasis where KLK8 influences inflammatory response. Research also explores its connection with the protein amyloid-beta contributing to an understanding of its involvement in neurodegenerative diseases. Understanding these interactions can further illuminate potential therapeutic targets for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.

See full target information KLK8

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 19:164 PubMed39578836

2024

Gastrodin attenuates diabetic cardiomyopathy characterized by myocardial fibrosis by inhibiting the KLK8-PAR1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

MingShan Zhang,YuFei Zhang,JingGang He,XinRui Wang,YinYin Wang,LinYan Li,Ling Tao,Min Zhang,Xiangchun Shen

International journal of molecular sciences 25: PubMed39273208

2024

Oral Administration of Probiotic Ameliorates Tonic-Clonic Seizure in a Pentylenetetrazole-Induced Kindling Mouse Model via Integrin-Linked Kinase Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiaki Ishii,Motohiro Kaya,Yoshikage Muroi

Discover oncology 15:324 PubMed39085670

2024

CCL28 promotes progression of hepatocellular carcinoma through PDGFD-regulated MMP9 and VEGFA pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Liu,Xingyi Chen,Xiading Zhang,Jingrou Guo,Yifei Tang,Cheng Jin,Minchen Wu

International journal of biological macromolecules 140:1126-1133 PubMed31449861

2019

Tissue kallikrein-related peptidase8 protects rat heart against acute ischemia reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Meineng Huang,Jiankui Du,Yifei Wang,Shiyu Ma,Ting Hu,Jing Shang,Qing Yu,Xiaoyan Zhu,Guanxin Zhang,Binhai Cong

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Kallikrein 8/KLK8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody (AB232839)

Western blot - Anti-Kallikrein 8/KLK8 antibody (AB232839)

Western blot - Anti-Kallikrein 8/KLK8 antibody (AB232839)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise