AB150395

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Kallikrein 8/KLK8 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

NRPN, PRSS19, TADG14, UNQ283/PRO322, KLK8, Kallikrein-8, hK8, Neuropsin, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, NP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling Kallikrein 8/KLK8 with ab150395 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

All lanes:

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Human fetal brain cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit

Predicted band size: 28 kDa

false

Carrier free

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5752(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 8 also known as KLK8 is a serine protease enzyme with a molecular mass of approximately 28 kDa. Alternative names include Neurosin and hK8. It is expressed in various tissues notably in the brain testis and skin. KLK8 plays a mechanical role in cleaving protein substrates in these regions aiding in maintaining cellular structures and processes through its proteolytic activity. Its expression is important in neuronal development making it significant in central nervous system function.

Biological function summary

KLK8 involves itself in tissue remodeling and neural plasticity. It interacts with extracellular matrix proteins contributing to cell migration and adhesion. Important in synaptic plasticity KLK8 supports memory formation and learning. KLK8 operates as part of proteolytic cascades and may overlap with other kallikreins in function. Its activity includes modulating neural and skin cells suggesting its importance in cellular differentiation and growth.

Pathways

KLK8 takes part in the kallikrein-kinin system and extracellular matrix degradation pathways. It interacts with proteins such as KLK3 (prostate-specific antigen) within these pathways although KLK3 is more associated with prostate tissue. KLK8's function in these pathways ensures proper maintenance and remodeling of tissue-specific extracellular environments. These activities highlight its relevance in maintaining physiological balance and cellular responses in its primary tissues of expression.

KLK8 links with neurological conditions and certain skin disorders. Altered levels or dysfunctions of KLK8 correlate with disorders like Alzheimer's disease indicating its potential role in amyloid processing. Similar associations exist with skin disorders like psoriasis where KLK8 influences inflammatory response. Research also explores its connection with the protein amyloid-beta contributing to an understanding of its involvement in neurodegenerative diseases. Understanding these interactions can further illuminate potential therapeutic targets for treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.

See full target information KLK8

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 24:53 PubMed38273291

2024

Tumor-derived KLK8 predicts inferior survival and promotes an immune-suppressive tumor microenvironment in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

He Tian,Ran Wei,Chu Xiao,Tao Fan,Yun Che,Tiejun Liu,Bo Zheng,Chunxiang Li,Jie He

Cell death & disease 12:860 PubMed34552064

2021

KLK8 promotes the proliferation and metastasis of colorectal cancer via the activation of EMT associated with PAR1.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Hua,Zhirong Sun,Yi Liu,Xuefang Shen,Weiwei Zhao,Xiaoyan Zhu,Pingbo Xu

Frontiers in oncology 11:624837 PubMed34395235

2021

Upregulation of KLK8 Predicts Poor Prognosis in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Hua,Tianjiao Li,Yixuan Liu,Xuefang Shen,Xiaoyan Zhu,Pingbo Xu

Theranostics 11:4207-4231 PubMed33754057

2021

A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Kui Du,Qing Yu,Yu-Jian Liu,Shu-Fang Du,Li-Yang Huang,Dan-Hong Xu,Xin Ni,Xiao-Yan Zhu

International journal of molecular sciences 21: PubMed32630820

2020

Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jaya Aseervatham,Kalu U E Ogbureke

Oncology reports 43:1705-1713 PubMed32323843

2020

Extracellular acidity‑induced expression of Kallikrein‑related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Chul Lim,Keun Hong Kee,Mi Ja Lee,Ran Hong,Song Iy Han

American journal of translational research 11:4180-4191 PubMed31396327

2019

Expression and clinical significance of KLK5-8 in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Lei,Qi Zhang,Fufen Yin,Xiangjun He,Jianliu Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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