JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154597

Anti-Kallistatin/PI-4 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal Kallistatin/PI-4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINA4 aa 100-400.

View Alternative Names

KST, PI4, SERPINA4, Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, Serpin A4, PI-4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Kallistatin/PI-4 antibody (AB154597)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kallistatin/PI-4 antibody (AB154597)

Immunofluorescent analysis of methanol-fixed HCT116 cells labeling Kallistatin/PI-4 with ab154597 at 1/500 dilution.
The image in the lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallistatin/PI-4 antibody (AB154597)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallistatin/PI-4 antibody (AB154597)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Kallistatin/PI-4 with ab154597 at 1/250 dilution.

Western blot - Anti-Kallistatin/PI-4 antibody (AB154597)
  • WB

Unknown

Western blot - Anti-Kallistatin/PI-4 antibody (AB154597)

10% SDS PAGE

All lanes:

Western blot - Anti-Kallistatin/PI-4 antibody (ab154597) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SERPINA4 aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

P29622

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol, using 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallistatin also known as PI-4 is a serine proteinase inhibitor with a mass of approximately 52 kDa. You find it mainly in the liver as part of the serpin superfamily. This protein acts mechanically by inhibiting the tissue kallikrein-kinin system which involves proteolytic processes that regulate vascular homeostasis and blood pressure. In addition to the liver kallistatin is expressed in endothelial cells and various human tissues.
Biological function summary

Kallistatin participates in inhibiting angiogenesis and inflammation impacting cellular behavior and responses. As a serpin it functions uniquely in forming stable complexes with target enzymes like tissue kallikrein. Through this mechanism kallistatin contributes to maintaining vascular integrity and preventing excessive vascular growth or inflammation that could lead to pathology.

Pathways

The inhibitory effects of kallistatin integrate into the kallikrein-kinin system and the renin-angiotensin system. In the kallikrein-kinin system kallistatin regulates processes involving vascular tone and fluid balance. It interacts with proteins such as bradykinin mediating inflammatory responses and endothelial cell function through these key pathways.

Researchers link kallistatin with conditions like hypertension and cancer. Its role in inhibiting angiogenesis and inflammation helps understand its protective effects against tumor growth and metastasis. In hypertension kallistatin's relationship with the renin-angiotensin system connects it to other proteins like angiotensin II influencing blood pressure regulation and vascular health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
See full target information SERPINA4
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412653 PubMed40091489

2025

Conditional Overexpression of Serpine2 Promotes Hair Cell Regeneration from Lgr5+ Progenitors in the Neonatal Mouse Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Hairong Xiao,Jiheng Wu,Lixuan Huang,Ying Ma,Leilei Wu,Yanqin Lin,Zixuan Ye,Xin Tan,Xujun Tang,Wei Tong,Mingchen Dai,Yintao Wang,Xia Sheng,Renjie Chai,Shasha Zhang

Journal of ovarian research 12:125 PubMed31884974

2019

Kallistatin inhibits tumour progression and platinum resistance in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wu,Rongrong Li,Zhiwei Zhang,Huiyang Jiang,Hanlin Ma,Cunzhong Yuan,Chenggong Sun,Yingwei Li,Beihua Kong
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB187656

Anti-Kallistatin/PI-4 antibody [EPR15310]

RabMAb|Recombinant

Western blot - Anti-Kallistatin/PI-4 antibody [EPR15310] (AB187656)

  • 0
  • 0

Complementary Products

Assay Kits

AB193714

Human Serpin A4 ELISA Kit

ELISA - Human Serpin A4 ELISA Kit (AB193714)

  • 0
  • 0
Primary Antibodies

AB183340

Anti-Nucleophosmin antibody [SP236]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] (AB183340)

  • 0
  • 0
Primary Antibodies

AB138491

Anti-GST3 / GST pi antibody [EPR8263]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] (AB138491)

  • 4
  • 1
Primary Antibodies

AB181548

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB181548)

  • 5
  • 4
Primary Antibodies

AB124767

Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (AB124767)

  • 5
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com