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AB250934

Anti-Kallistatin/PI-4 antibody [EPR15310] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Kallistatin/PI-4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KST, PI4, SERPINA4, Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, Serpin A4, PI-4

1 Images
Western blot - Anti-Kallistatin/PI-4 antibody [EPR15310] - BSA and Azide free (AB250934)
  • WB

Supplier Data

Western blot - Anti-Kallistatin/PI-4 antibody [EPR15310] - BSA and Azide free (AB250934)

This data was developed using ab187656, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Kallistatin/PI-4 antibody [EPR15310] (<a href='/en-us/products/primary-antibodies/kallistatin-pi-4-antibody-epr15310-ab187656'>ab187656</a>) at 1/1000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15310

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250934 is the carrier-free version of ab187656.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallistatin also known as PI-4 is a serine proteinase inhibitor with a mass of approximately 52 kDa. You find it mainly in the liver as part of the serpin superfamily. This protein acts mechanically by inhibiting the tissue kallikrein-kinin system which involves proteolytic processes that regulate vascular homeostasis and blood pressure. In addition to the liver kallistatin is expressed in endothelial cells and various human tissues.
Biological function summary

Kallistatin participates in inhibiting angiogenesis and inflammation impacting cellular behavior and responses. As a serpin it functions uniquely in forming stable complexes with target enzymes like tissue kallikrein. Through this mechanism kallistatin contributes to maintaining vascular integrity and preventing excessive vascular growth or inflammation that could lead to pathology.

Pathways

The inhibitory effects of kallistatin integrate into the kallikrein-kinin system and the renin-angiotensin system. In the kallikrein-kinin system kallistatin regulates processes involving vascular tone and fluid balance. It interacts with proteins such as bradykinin mediating inflammatory responses and endothelial cell function through these key pathways.

Researchers link kallistatin with conditions like hypertension and cancer. Its role in inhibiting angiogenesis and inflammation helps understand its protective effects against tumor growth and metastasis. In hypertension kallistatin's relationship with the renin-angiotensin system connects it to other proteins like angiotensin II influencing blood pressure regulation and vascular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
See full target information SERPINA4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3728 PubMed37349339

2023

NBEAL2 deficiency in humans leads to low CTLA-4 expression in activated conventional T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Delage,Francesco Carbone,Quentin Riller,Jean-Luc Zachayus,Erwan Kerbellec,Armelle Buzy,Marie-Claude Stolzenberg,Marine Luka,Camille de Cevins,Georges Kalouche,Rémi Favier,Alizée Michel,Sonia Meynier,Aurélien Corneau,Caroline Evrard,Nathalie Neveux,Sébastien Roudières,Brieuc P Pérot,Mathieu Fusaro,Christelle Lenoir,Olivier Pellé,Mélanie Parisot,Marc Bras,Sébastien Héritier,Guy Leverger,Anne-Sophie Korganow,Capucine Picard,Sylvain Latour,Bénédicte Collet,Alain Fischer,Bénédicte Neven,Aude Magérus,Mickaël Ménager,Benoit Pasquier,Frédéric Rieux-Laucat
View all publications
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Product promise

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