AB60018

Anti-kan antibody [4B4D1]

(1 Review)

(13 Publications)

Mouse Monoclonal KKA8 antibody 4B4D1. Suitable for ELISA, WB and reacts with Recombinant fragment, Recombinant full length protein - Escherichia coli samples. Cited in 13 publications.

View Alternative Names

APH(3') II, Aminoglycoside 3' phosphotransferase, EC 2.7.1.95, Kan, Kanamycin kinase type II, Kanamycin kinase, type VIII, NPT II, Neo, Neomycin Phosphotransferase 2, Neomycin kanamycin phosphotransferase type II, Neomycin kanamycin phosphotransferase type VIII, Neomycin phosphotransferase II, Neomycine phosphotransferase, aphA, kanamycin resistance gene

1 Images

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Western blot - Anti-kan antibody [4B4D1] (AB60018)

All lanes:

Western blot - Anti-kan antibody [4B4D1] (ab60018) at 1/500 dilution

All lanes:

Recombinant kan expressed in E. coli.

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B4D1

Isotype

IgG2a

Carrier free

Applications

ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Escherichia coli": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAN is a biological target that commonly refers to kanamycin resistance which is represented by genes such as 'kan'. This target is also known as neomycin phosphotransferase (also called 'neo kan' or 'antikan') and typically has a molecular mass around 30 kDa. It facilitates resistance to the antibiotic kanamycin by phosphorylating the drug rendering it inactive. Gene 'kan' is often part of plasmids used in laboratory strains of E. coli and other bacteria for selection purposes.

Biological function summary

By conferring resistance to kanamycin the kan gene enables the proliferation of only those cells in a culture that have incorporated the gene of interest. This selectable marker is often integrated into the host cells alongside the gene of interest during genetic engineering processes. It does not function independently but often pairs with other genes in plasmid constructs used in various biological studies.

Pathways

The kan gene plays an indirect role as it is not directly involved in metabolic or signaling pathways like more traditional enzymes or proteins. However it interacts with the antibiotic resistance pathway impacting bacterial regulatory networks associated with drug resistance. The expression of kan is important within plasmid-based cloning vectors and stands alongside other resistance markers such as the ampicillin resistance protein influencing the overall effectiveness of gene manipulation techniques.

Kan gene-related studies often intersect with research focused on antibiotic resistance which poses significant challenges in treating bacterial infections. While kan itself does not directly cause diseases the prevalence of antibiotic resistance genes like kan in pathogenic bacteria contributes to the complexities surrounding multi-drug-resistant infections. This scenario highlights the relevance of proteins such as the multi-drug efflux pump often found in resistant strains which further complicates the treatment of such infections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information kan

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107752 PubMed37954141

2023

The PtdIns3P phosphatase MtMP promotes symbiotic nitrogen fixation via mitophagy in .

Applications

Unspecified application

Species

Unspecified reactive species

Qixia Xue,Chen Shen,Qianwen Liu,Peng Liu,Da Guo,Lihua Zheng,Jinling Liu,Chang Liu,Qinyi Ye,Tao Wang,Jiangli Dong

Nature communications 14:2807 PubMed37198152

2023

The thioesterase APT1 is a bidirectional-adjustment redox sensor.

Applications

Unspecified application

Species

Unspecified reactive species

Tuo Ji,Lihua Zheng,Jiale Wu,Mei Duan,Qianwen Liu,Peng Liu,Chen Shen,Jinling Liu,Qinyi Ye,Jiangqi Wen,Jiangli Dong,Tao Wang

Autophagy 16:862-877 PubMed31362589

2019

Dehydrin MtCAS31 promotes autophagic degradation under drought stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Qianwen Liu,Hao Feng,Jie Deng,Rongxue Zhang,Jiangqi Wen,Jiangli Dong,Tao Wang

Scientific reports 7:40388 PubMed28091612

2017

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Beuchert Kallehauge,Shangzhong Li,Lasse Ebdrup Pedersen,Tae Kwang Ha,Daniel Ley,Mikael Rørdam Andersen,Helene Faustrup Kildegaard,Gyun Min Lee,Nathan E Lewis

Journal of Alzheimer's disease : JAD 42:1221-7 PubMed25024306

2014

Bridging integrator 1 (BIN1) protein expression increases in the Alzheimer's disease brain and correlates with neurofibrillary tangle pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Holler,Paulina R Davis,Tina L Beckett,Thomas L Platt,Robin L Webb,Elizabeth Head,M Paul Murphy

The Journal of general virology 95:2427-2441 PubMed25024280

2014

NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Bentham,Najat Marraiki,Christopher J McCormick,David J Rowlands,Stephen Griffin

Proceedings of the National Academy of Sciences of 110:18238-43 PubMed24145442

2013

Therapeutic potential of proteasome inhibitors in congenital erythropoietic porphyria.

Applications

Species

Mouse

Jean-Marc Blouin,Yann Duchartre,Pierre Costet,Magalie Lalanne,Cécile Ged,Ana Lain,Oscar Millet,Hubert de Verneuil,Emmanuel Richard

PloS one 8:e71332 PubMed23936500

2013

Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture.

Applications

IHC-P

Species

Zebrafish

Ten-Tsao Wong,Paul Collodi

PloS one 8:e62660 PubMed23671620

2013

Production of zebrafish offspring from cultured female germline stem cells.

Applications

IHC - Wmt, IHC-FoFr

Species

Zebrafish, Zebrafish

Ten-Tsao Wong,Abraham Tesfamichael,Paul Collodi

Nucleic acids research 40:4998-5011 PubMed22323517

2012

The DEAD-box helicase DDX3 supports the assembly of functional 80S ribosomes.

Applications

Species

Human

Rene Geissler,Ralph P Golbik,Sven-Erik Behrens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-kan antibody [4B4D1]

Western blot - Anti-kan antibody [4B4D1] (AB60018)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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