Anti-kan antibody [4B4D1]

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KAN is a biological target that commonly refers to kanamycin resistance which is represented by genes such as 'kan'. This target is also known as neomycin phosphotransferase (also called 'neo kan' or 'antikan') and typically has a molecular mass around 30 kDa. It facilitates resistance to the antibiotic kanamycin by phosphorylating the drug rendering it inactive. Gene 'kan' is often part of plasmids used in laboratory strains of E. coli and other bacteria for selection purposes.

Biological function summary

By conferring resistance to kanamycin the kan gene enables the proliferation of only those cells in a culture that have incorporated the gene of interest. This selectable marker is often integrated into the host cells alongside the gene of interest during genetic engineering processes. It does not function independently but often pairs with other genes in plasmid constructs used in various biological studies.

Pathways

The kan gene plays an indirect role as it is not directly involved in metabolic or signaling pathways like more traditional enzymes or proteins. However it interacts with the antibiotic resistance pathway impacting bacterial regulatory networks associated with drug resistance. The expression of kan is important within plasmid-based cloning vectors and stands alongside other resistance markers such as the ampicillin resistance protein influencing the overall effectiveness of gene manipulation techniques.

Associated diseases and disorders

Kan gene-related studies often intersect with research focused on antibiotic resistance which poses significant challenges in treating bacterial infections. While kan itself does not directly cause diseases the prevalence of antibiotic resistance genes like kan in pathogenic bacteria contributes to the complexities surrounding multi-drug-resistant infections. This scenario highlights the relevance of proteins such as the multi-drug efflux pump often found in resistant strains which further complicates the treatment of such infections.