Rabbit Polyclonal KANK2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KANK2 aa 550-600.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: PBS, Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KANK2 aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q63ZY3
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 (PubMed:17476305). Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria (PubMed:22371500). Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis (PubMed:22371500). Involved in the negative control of vitamin D receptor signaling pathway (PubMed:24671081). Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway (PubMed:17996375, PubMed:25961457). May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney (PubMed:25961457). Through the Rho signaling pathway may also regulate cell proliferation (By similarity).
Alternative names
ANKRD25, KIAA1518, MXRA3, SIP, KANK2, KN motif and ankyrin repeat domain-containing protein 2, Ankyrin repeat domain-containing protein 25, Matrix-remodeling-associated protein 3, SRC-1-interacting protein, SRC-interacting protein, SRC1-interacting protein
Recommended products
Rabbit Polyclonal KANK2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KANK2 aa 550-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: PBS, Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KANK2 aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q63ZY3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The KANK2 protein or KN motif and ankyrin repeat domains 2 plays a role in modulating the cytoskeleton by interacting with the actin network. It weighs around 86 kDa. KANK2 is expressed in various tissues with notable levels in the kidneys and testes. Its location in the cell membrane allows it to contribute to maintaining cellular structure and enabling intracellular communication.
- Biological function summary
KANK2 interacts with multiple proteins and is a part of a protein complex involved in cytoskeletal organization. It functions by modulating microtubule dynamics and stabilizing cell-cell junctions impacting cellular adhesion and migration processes. The ankyrin repeat domains in KANK2 facilitate these interactions which are essential for the integrity and function of cellular structures.
- Pathways
KANK2 participates in signaling pathways related to cytoskeleton rearrangement and cell adhesion. It interacts with proteins such as Rho GTPases that regulate these pathways. Through these interactions KANK2 plays a role in controlling cell shape and motility which are significant in processes like wound healing and embryonic development.
- Associated diseases and disorders
Alterations in KANK2 expression or function have associations with diseases like nephrotic syndrome and some cancers. In nephrotic syndrome KANK2 mutations can disrupt the kidney filtration barrier leading to the condition. Its interaction with proteins such as nephrin highlights its role in maintaining podocyte function which is important in kidney health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK2 antibody (ab99351)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-KANK2 antibody (ab99351)
Detection of Human KANK2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate (lane 1). KANK2 was also immunoprecipitated by another rabbit anti-KANK2 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.
All lanes: Immunoprecipitation - Anti-KANK2 antibody (ab99351)
Predicted band size: 91 kDa
Western blot - Anti-KANK2 antibody (ab99351)
All lanes: Western blot - Anti-KANK2 antibody (ab99351) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3min
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