AB99351

Anti-KANK2 antibody

(1 Review)

(1 Publication)

Rabbit Polyclonal KANK2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KANK2 aa 550-600.

View Alternative Names

ANKRD25, KIAA1518, MXRA3, SIP, KANK2, KN motif and ankyrin repeat domain-containing protein 2, Ankyrin repeat domain-containing protein 25, Matrix-remodeling-associated protein 3, SRC-1-interacting protein, SRC-interacting protein, SRC1-interacting protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK2 antibody (AB99351)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK2 antibody (AB99351)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ANKRD25 with ab99351 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-KANK2 antibody (AB99351)

Detection of Human KANK2 by Western Blot and Immunoprecipitation. Samples : Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate (lane 1). KANK2 was also immunoprecipitated by another rabbit anti-KANK2 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.

All lanes:

Immunoprecipitation - Anti-KANK2 antibody (ab99351)

Predicted band size: 91 kDa

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Western blot - Anti-KANK2 antibody (AB99351)

All lanes:

Western blot - Anti-KANK2 antibody (ab99351) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 91 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human KANK2 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q63ZY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, PBS

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KANK2 protein or KN motif and ankyrin repeat domains 2 plays a role in modulating the cytoskeleton by interacting with the actin network. It weighs around 86 kDa. KANK2 is expressed in various tissues with notable levels in the kidneys and testes. Its location in the cell membrane allows it to contribute to maintaining cellular structure and enabling intracellular communication.

Biological function summary

KANK2 interacts with multiple proteins and is a part of a protein complex involved in cytoskeletal organization. It functions by modulating microtubule dynamics and stabilizing cell-cell junctions impacting cellular adhesion and migration processes. The ankyrin repeat domains in KANK2 facilitate these interactions which are essential for the integrity and function of cellular structures.

Pathways

KANK2 participates in signaling pathways related to cytoskeleton rearrangement and cell adhesion. It interacts with proteins such as Rho GTPases that regulate these pathways. Through these interactions KANK2 plays a role in controlling cell shape and motility which are significant in processes like wound healing and embryonic development.

Alterations in KANK2 expression or function have associations with diseases like nephrotic syndrome and some cancers. In nephrotic syndrome KANK2 mutations can disrupt the kidney filtration barrier leading to the condition. Its interaction with proteins such as nephrin highlights its role in maintaining podocyte function which is important in kidney health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 (PubMed : 17476305). Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria (PubMed : 22371500). Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis (PubMed : 22371500). Involved in the negative control of vitamin D receptor signaling pathway (PubMed : 24671081). Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway (PubMed : 17996375, PubMed : 25961457). May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney (PubMed : 25961457). Through the Rho signaling pathway may also regulate cell proliferation (By similarity).

See full target information KANK2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1578163 PubMed40384984

2025

Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growth.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Liu,Ningjian Cai,Youchao Dai,Xinchun Chen,Xiaobin Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-KANK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK2 antibody (AB99351)

Immunoprecipitation - Anti-KANK2 antibody (AB99351)

Western blot - Anti-KANK2 antibody (AB99351)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise